Fas／FasL及增殖细胞核抗原表达与人黑素瘤恶性程度  

The relationship between Fas/FasL and proliferating cell nudear antigen expression and malignant degree in human melanoma

作　　者：王永晨[1] 谭岩[1] 

机构地区：[1]吉林大学第一医院生物治疗中心,长春130021

出　　处：《中华医学杂志》2008年第14期965-968,共4页National Medical Journal of China

基　　金：黑龙江省卫生厅资助课题基金资助项目（2007-247）

摘　　要：目的探讨Fas和Fas配体（Fas／FasL）及增殖细胞核抗原（PCNA）在人黑素瘤中表达情况及其与黑素瘤恶性程度的关系。方法按不同病理分级，将标本分为恶性黑素瘤（MM）组、黑素细胞痣组和正常皮肤对照组，应用免疫组化SP法，检测Fas／FasL及PCNA在各组标本中表达情况。结果Fas、FasL及PCNA在三组中表达强度差异有统计学意义（P〈0．01）；MM组PCNA阳性细胞指数（PI）（55．0％±14．8％）明显高于正常皮肤组（4．0％±2．0％）和黑素细胞痣组（6．2％±3．0％）（P〈0．01），而后二者间差异则无统计学意义（P〉0．05）。随着PI增加MM浸润深度增加、伴有淋巴结转移可能性明显增加、肿瘤细胞分化程度越差、恶性度越高（P〈0．01）；但是PI与MM病理分型之间没有直接量效关系（P〉0．05）。结论Fas／FasL及PCNA表达情况反映人黑素瘤恶性程度，PCNA表达越强，恶性度越高；Fas／FasL及PCNA表达与黑素瘤发展过程密切联系。Objective To study the expression of Fas, Fas-ligand （Fas/FasL）, and proliferating cell nuclear antigen （PCNA） in malignant melanoma （MM）, melanocytic nevus, and normal skin, and the relationship of Fas, Fas/FasL, and PCNA expressions to malignant degrees of MM. Methods Immunohistochemistry SP methods was used to detect the expressions of Fas, Fas-ligand （ Fas/FasL）, and PCNA in 35 samples of MM, 21 samples of melanocytic nevus, and 28 normal skin samples obtained by operation. Results The positive Fas rate of the MM group was 92.86% （26/28）, significantly higher than those of the melaoncytic nevus group （76.19% , 16/21 ） and normal skin group （45.71% , 16/35 ） （Χ^2 = 16.84,v=2,P 〈0.01 ）. The positive FasL rate of the MM group was 3.57% （ 1/28）, significantly lower than those of the those of the melaoncytic nevus group （19.05%, 4/21 ） and normal skin group （62.86%, 22/35） （Χ^2 =27.27, v =2,P 〈0.01）. The PCNA positive rate of the MM group was 100% （35/35） , significantly higher than those of the melaoncytic nevus group （ 33.33%, 7/21 ） and normal skin group （10.71%, 3/28） （Χ^2 =54.4,v =2,P 〈0.01）. The positive index （PI） of PCNA of the MM group was 55.0% ± 14.8% , significantly higher than those of the melaoncytic nevus group （ 6.2% ± 3.0% , t = 19. 89,P〈0.01） and normal skin group （4.0% ±2.0% , t =22.63,P 〈0. 01）, however, there was no significant difference in the PCNA PI between the melaoncytic nevus and normal skin groups （ P 〉 0.05 ）. The greater the infiltration depth of MM, the higher the PCNA PI. However, there was no direct dose-effect relationship between PI and pathological types. Conclusion The expressions of Fas/FasL and PCNA reflect the malignant degree of human melanoma, and the more expression of PCNA, the more malignant degree of melanoma. The expressions of Fas/FasL and PCNA are closely associated with the development of MM.

关 键 词：黑色素瘤 增殖细胞核抗原 FAS/FAS配体 免疫组化 

分 类 号：R686[医药卫生—骨科学]