肝癌化疗栓塞术后残癌和癌旁组织血管内皮生长因子受体2及其磷酸化状态的表达水平  

Vascular endothelial growth factor receptor-2 and its phosphorylated state in cancerous and peri- cancerous tissues of hepatocellular carcinoma after transcatheter arterial

作　　者：周大勇[1] 王建华[1] 刘嵘[1] 钱晟[1] 王平[1] 颜志平[1] 

机构地区：[1]复旦大学附属中山医院放射科,上海200032

出　　处：《中华放射学杂志》2008年第4期406-409,共4页Chinese Journal of Radiology

摘　　要：目的了解肝癌经动脉化疗栓塞术后残癌及癌旁组织的血管内皮生长因子受体2（VEGFR-2）表达水平及其磷酸化状态水平的差别和意义。方法取经导管肝动脉化疗栓塞术（TACE）后行二期手术切除的肝癌及其周围组织标本43例份（TACE加手术组）和未经任何治疗直接手术切除的肝癌及周围组织标本20例份（单纯手术组），用免疫组织化学染色方法，检测残癌及癌旁组织VEGFR-2、磷酸化VEGFR-2、缺氧诱导因子1α[（HIF-1α）的表达水平和微血管密度[MVD（CD31标记）]；采用方差分析及Χ^2检验比较2组间各指标的差异。结果TACE加手术组残癌组织磷酸化VEGFR-2和HIF-1α的吸光度值（A值）分别为（0．034±0．016）、（0．047±0．021），单纯手术组分别为（0．024±0．009）、（0．035±0．016），2组间差异均有统计学意义（F值分别为6．75、4．77，P值均〈0．05）；TACE加手术组癌旁组织VEGFR-2、磷酸化VEGFR-2和HIF-1α的A值分别为（0．040±0．017）、（0．031±0．011）和（0．037±0．015），单纯手术组分别为（0．030±0．015）、（0．020±0．008）和（0．024±0．014），2组间差异均有统计学意义（F值分别为4．60、13．72、11．65，P值均〈0．05）；TACE加手术组和单纯手术组的癌旁组织MVD分别为（58．3±15．2）、（44．4±10．5）个／高倍视野，2组差异有统计学意义（Χ^2=13．64，P〈0．05）。结论肝癌经动脉化疗栓塞术后残癌组织与癌旁组织缺氧程度加重，VEGFR-2表达增加且功能增强。Objective To investigate vascular endothelial growth factor receptor-2 （VEGFR-2） and its phosphorylated state in cancerous and peri-cancerous tissues of hepatocellular carcinoma （HCC） after transcatheter arterial chemoembolization（TACE）. Methods Forty-three cases of second-stage surgery of HCC after TACE （ TACE ＆ surgery group） and twenty cases of surgery without TACE （ direct surgery group） were performed. Surgical specimens were made into paraffin-embedded slices. VEGFR-2, phosphorylated VEGFR-2, hypoxia induced factor-1α （HIF-1α） and microvessel density （MVD, labeled by CD31 ） were detected using immunohistochemical method. All data was statistically processed with SPSS for Windows. Results The absorption factor（A factor） of phosphorylated VEGFR-2 and HIF-1 α in the cancerous tissue of HCC were （0. 034 ± 0. 016） and （0. 047 ± 0. 021 ） in TACE ＆ surgery group, and （ 0. 024 ± 0. 009 ） and （0. 035 ±0. 016）in direct-surgery group, which showed statistically significant difference（F = 6. 75,4. 77, P 〈0.05）. The A factors of VEGFR-2, phosphorylated VEGFR-2 and HIF-1α in the peri-cancerous tissue were （0.040±0.017）, （0.031 ±0.011） and （0.037 ±0.015） respectively in TACE ＆ surgery group, and（0. 030 ±0. 015） , （0. 020 ±0. 008） and （0. 024 ± 0. 014） respectively in direct-surgery group, all of which showed statistically significant difference （ F = 4.60, 13.72, 11.65, P 〈 0. 05 ）. MVD of the peri- cancerous tissue was （ 58. 3 ± 15.2 ）/HP in TACE ＆ surgery group and （44.4 ± 10. 5 ）/HP in direct-surgery group, which showed statistically significant difference （ Χ^2 = 13.64, P 〈 0. 05 ） . Conclusion The cancerous and peri-cancerous tissues in HCC after TACE showed more hypoxic changes. Both expression and function of VEGFR-2 were enhanced following TACE.

关 键 词：癌 肝细胞 化学栓塞 治疗性 血管内皮生长因子受体2 放射学 介入性 

分 类 号：R686[医药卫生—骨科学]