利用高分子量麦谷蛋白亚基电泳图谱鉴定小麦新品种的特异性  

Identification on New Varieties of Wheat by High Molecular Weight Glutenin Subunit

作　　者：李群[1] 王建成[1] 颜廷进[1] 张文兰[1] 段乃彬[1] 戴双[1] 

机构地区：[1]农业部农作物种质监督检验测试中心(济南),山东济南250100

出　　处：《种子》2008年第3期17-21,共5页Seed

摘　　要：根据《小麦植物新品种测试指南》的要求,本研究应用SDS-PAGE电泳方法,对79个测试品种及其近似品种进行了高分子量麦谷蛋白亚基分析。结果表明,申请品种和近似品种的高分子量麦谷蛋白亚基存在着丰富的变异,在Glu-A1、Glu-B1、Glu-D1三个位点有12种变异类型,共检测出17个不同类型的亚基组合。利用这一性状,能够将约占69.6%的测试品种与近似品种区分开。和形态学性状相比,麦谷蛋白高分子量亚基表达状态多,表达稳定,遗传机制明确,可作为小麦品种测试中重要的高辨别力性状。High molecular weight glutenin subunit （HMW-GS） in 177 wheat applicant varieties and reference varieties were analyzed by SDS-PAGE based on Guidelines for the Conduct of distinctness, uniformity and stability （DUS） for Wheat （ Triticum aestivum L. ）. High variation for HMW-GS was found among applicant varieties and reference varieties. 12 alleles were detected at Glu-A 1 ,Glu-B 1 and Glu-D 1 ,and 17 subunit combinations were identified. There were 69.6% applicant varieties which cauld be distinguished from their reference varieties. Compared with morphological characters, HMW-GS is an ideal character for DUS testing because of its polymorphism of states,stable expression and clear genetic interpretation.

关 键 词：小麦 高分子量麦谷蛋白亚基(HMW-GS) 品种鉴定 DUS测试 新品种保护 

分 类 号：S512.1[农业科学—作物学]