32例有偿献血感染者HIV-1膜区序列测定及V3环氨基酸变异特点分析  

Analysis of the variation of 32 HIV-1 V3 loop from infected Chinese blood/plasma donors

作　　者：代丽丽[1] 鲁俊峰[1] 黄晓婕[1] 石英[1] 陈德喜[1] 吴昊[1] 

机构地区：[1]首都医科大学附属北京佑安医院感染科

出　　处：《中华实验和临床病毒学杂志》2008年第1期27-29,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：基金项目：北京市科委科技计划重大项目（D0906003040591）;国家高技术研究发展计划（863计划）（2006AA02Z411）.

摘　　要：目的了解我国有偿献血人群中HIV-1B’亚型流行株的膜蛋白v3环序列特征。方法巢式PCR扩增前病毒DNAc2-c3区后测序，进行病毒亚型鉴定和V3环序列特点分析。结果所检的32个HIV-1B’亚型株v3环顶端四肽有五种形式。AIDS组与无症状感染组相比前者呈现更多的T/SI型毒株V3环变异特点。结论我国有偿献血人群中HIV-1B’亚型流行株V3环顶端四肽有多种形式。处于病程不同时期的患者体内病毒V3区呈现不同的氨基酸变异特点和电荷积累趋势，提示可能具有不同的生物学表型特征。Objective To characterize the variation in V3 loop of HIV-1 B＇ strains circulating in Chinese blood donors. Methods The c2-c3 regions of the HIV envelop gene were amplified by nest-PCR from 32 HIV-1-infected blood donors in He Nan province in China. The BIOEDIT and MEGA software are used to analyze the sequences of V3 loop. Results There were five types of central motifs of the 32 samples, in which GPGR and GPGQ are most common. More variations associated with T tropic/SI phenotype can be seen in AIDS group. Conclusion The V3 tip motifs of HIV-1B＇ strains circulating in Chinese blood donors are various. The different characterization of V3 loop between AIDS and asymptomatic patients indicates different biological phenotype and pathogenesis which warrant additional investigation.

关 键 词：HIV-1 基因型 序列分析 生物学表型 

分 类 号：R450[医药卫生—治疗学]