慢性乙型肝炎患者肝组织内诱导型一氧化氮合酶的表达与肝组织病变的关系  

Increased expression of inducible nitric oxide synthase in chronic hepatitis B patients is correlated with histopathological grading and staging

作　　者：吴娟[1] 王凯[1] 韩利岩[1] 范玉琛[1] 

机构地区：[1]山东大学齐鲁医院肝病科,山东济南250012

出　　处：《中华实验和临床病毒学杂志》2008年第1期57-59,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的探讨诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）在慢性乙型肝炎（chronic hepatitis B，CHB）患者肝组织内的表达及与肝组织病变的关系和临床意义。方法应用免疫组织化学法检测74例CHB患者肝组织内iNOS的表达和分布情况，并分析其与患者肝功能、HBeAg、HBV DNA及肝组织病理分级和分期的关系。结果CHB患者组与正常对照组相比，肝组织内iNOS表达明显增加（P〈0．01）。CHB患者肝组织内iNOS表达强度与ALT水平、肝组织炎症程度和纤维化分期之间呈显著正相关（r=0.601，P=0.000；r=0.660，P=0.000；r=0.507，P=0.000）。结论CHB患者肝组织内iNOS表达显著增高，并随着肝脏炎症和纤维化程度的加重表达增强，与CHB病变进展密切相关。Objective To investigate the intrahepatic expression of inducible nitric oxide synthase （iNOS） in patients with chronic hepatitis B （CHB） and its relation to liver histopathology. Methods The intensity and distribution of the immunohistochemical staining of intrahepatic iNOS were studied in the liver biopsy specimens obtained from 74 patients with CHB and statistical analyses were performed between intrahepatic iNOS and ALT, HBeAg, HBV DNA, grading of liver inflammation and staging of fibrosis. Seven histologically normal liver sections were used as a control group. Results Compared with the control group, the intrahepafie iNOS immunoexpression was significantly higher in patients with CHB （ P 〈 0.05 ）. iNOS immunoreaetivity was observed mainly in hepatocytes, showing a predominant cytoplasmic staining, with the positive liver cells distributed diffusely throughout the hepatic lobule. Immunopositive staining could also be detected in Kupffer cells, sinuseidal lining cells and vascular endothelial cells. Compared with patients with normal ALT, the hepatoeellular iNOS immunoexpression was significantly higher in patients with elevated ALT （P 〈 0.05） and the iNOS immunoexpression was significantly correlated with the serum level of ALT （r=0.601, P= 0.000）. Statistical analysis also showed that the intrahepatie iNOS immunoexpression was positively correlated with the grading of liver inflammation and the staging of liver fibrosis （r = 0.660, P = 0.000; r = 0.507, P = 0.000）. No significant correlation between iNOS and HBeAg and HBV DNA was detected. Conclusion The intrahepatie expression of iNOS is elevated in chronic hepatitis B patients and correlated well with the severity of the disease, which indicated that inducible nitric oxide synthase may have a critical role in the pathogenesis of chronic viral hepatitis B.

关 键 词：一氧化氮合酶 肝炎 乙型 慢性 免疫组织化学 

分 类 号：R285.5[医药卫生—中药学]