LC-MS/MS法测定人血浆中倍他米松(英文)  

Determination of betamethasone in human plasma by liquid chromatography with tandem mass

作　　者：曲婷婷[1] 张蕊[1] 王本杰[1] 刘晓燕[1] 袁桂艳[1] 郭瑞臣[1] 

机构地区：[1]山东大学齐鲁医院临床药理研究所,山东济南250012

出　　处：《药学学报》2008年第4期402-407,共6页Acta Pharmaceutica Sinica

摘　　要：建立测定人血浆中倍他米松的LC—MS／MS方法。采用Venusil XBP C8（200mm×3．9mmID，5μm）色谱柱，流动相为甲醇-水（含甲酸铵5mmol·L^-1）（80：20），流速0．4mL·min^-1；质谱仪离子源为电喷雾离子源（ESI），正离子模式检测，监测离子为393．3→355．2（倍他米松）和361．3→343．2（泼尼松龙，内标）。血浆样本用乙酸乙酯处理。倍他米松在0．5—80．0ng·mL^-1线性关系良好（r=0．9992），血浆低、中、高3种浓度（1．0，10．0，60．0ng·mL^-1）平均提取回收率为88．24％，定量限为0．5ng·mL^-1。本方法操作简便、准确、灵敏，适用于复方倍他米松注射液人体药代动力学研究。A sensitive and selective liquid chromatography-tandem mass spectrometric （LC-MS/MS） method was developed and validated for the determination of hetamethasone in human plasma. The analyte was isocratically eluted on a Venusil XBP Cs column （200 mm × 3.9 mm ID, 5μm） with methanol-water （containing 5 mmol ·L^-l ammonium formate） （80： 20） at a flow rate of 0.4ng · min^-1 and detected with a triple quad LC-MS/MS using ESI with positive ionization. Ions monitored in the multiple reaction monitoring （MRM） mode were m/z 393.3 → 355.2 for betamethasone and m/z 361.3 →343.2 for prednisolone （IS）. Betamethasone was extracted from 0. 5 mL human plasma with ethyl acetate. The average recovery is 88.24% and the low limit of quantitation （LLOQ） was 0.5 ng · mL^-1. The 3-day validation study demonstrated excellent precision and accuracy across the calibration range of 0.5 - 80. 0 ng · mL^-1. The method was successfully applied to the pharmacokinetic study of compound hetamethason injection in healthy Chinese volunteers.

关 键 词：倍他米松 LC-MS/MS 药代动力学 复方倍他米松注射液 

分 类 号：R917[医药卫生—药物分析学]