复方鳖甲软肝方药物血清对大鼠肝星形细胞内TβR Ⅰ和Smad_3蛋白表达的影响实验研究  

EXPERIMENTAL STUDY OF FUFANGBIEJIARUANGANFANG DRUG SERUM ON THE EXPRESSION OF TGF-β TYPE Ⅰ RECEPTOR AND Smad_3 PROTEIN IN RAT HSCs

作　　者：郭顺根[1] 谢艳爽[1] 张玮[1] 贾文婷[1] 赵海军[1] 庞奕晖[1] 戚红丹[1] 

机构地区：[1]北京中医药大学细胞与生化实验室,北京100029

出　　处：《解剖学报》2008年第2期180-186,共7页Acta Anatomica Sinica

基　　金：教育部长江学者和创新团队发展计划(IRT0413);国家重点研究发展计划973课题(2007CB512705)资助

摘　　要：目的观察复方鳖甲软肝方药物血清对大鼠肝星形细胞(HSCs)内TβRⅠ和Smad3蛋白表达的影响,从信号转导上游受体水平和受体后信号转导水平探讨药物血清抗肝纤维化的作用机制。方法运用免疫细胞化学和图像分析技术,在离体对不同时相,不同浓度复方鳖甲软肝方药物血清干预状态下,HSC内TβRⅠ和Smad3蛋白的表达进行半定量分析。结果复方鳖甲软肝方药物血清可明显下调HSC表达TβRⅠ及抑制HSC表达Smad3蛋白。结论复方鳖甲软肝方药物血清通过下调TβRⅠ的表达,进而干预Smad3介导的TGF-β1在HSC内的信号转导,最终抑制胶原蛋白、纤黏连蛋白的合成,从而达到抑制HSC活化的抗肝纤维化作用,这可能是复方鳖甲软肝方药物血清抗肝纤维化作用机制之一。Objective To investigate the effect of fufangbiejiaruanganfang drug serum on the expression of TGF-β type I receptor and Smad_3 protein in rat hepatic stellate cells（HSCs）.Methods Immunocytochemical staining and image analysis were applied to investigate the effect of different dosages of fufangbiejiaruanganfang drug serums on the expression of TGF-β type I receptor and Smad_3 protein in rat HSCs.Results High and medium dosage of fufangbiejiaruanganfang drug serum remarkably downregulated the expression of TGF-β type I receptor and Smad_3 protein in rat HSCs in a dose-dependent manner.Conclusion The experiment demonstrated that fufangbiejiaruanganfang drug serums can downregulate the expression of TGF-β type I receptor and Smad_3 protein in HSCs and further inhibit the activation of HSCs and improve liver fibrosis.So the downregulation of TGFβ type I receptor in HSCs may be one of the mechanisms of fufangbiejiaruanganfang against liver fibrosis.

关 键 词：复方鳖甲软肝方 肝星形细胞 转化生长因子Β1 SMAD 蛋白 免疫组织化学 大鼠 

分 类 号：R289.5[医药卫生—方剂学]