卵巢切除对大鼠肾上腺雌激素受体亚型表达的影响  

EFFECT OF OVARIECTOMY ON THE EXPRESSION OF ESTROGEN RECEPTER SUBTYPE IN THE ADRENAL GLANDS OF THE RATS

作　　者：薛晓鸥[1] 师莉莉[2] 牛建昭[3] 艾浩[3] 杨毅[2] 李键[3] 

机构地区：[1]北京中医药大学东方医院妇科,北京100078 [2]辽宁医学院附属第一医院妇产科,锦州121001 [3]北京中医药大学组织学与胚胎学教研室,北京100029

出　　处：《解剖学报》2008年第2期223-226,共4页Acta Anatomica Sinica

基　　金：教育部长江学者和创新团队发展计划(RT0413);教育部高等学校学科创新引智计划(B07007)资助

摘　　要：目的通过对去卵巢大鼠雌激素受体(ER)亚型在肾上腺表达的观察,研究去卵巢对大鼠肾上腺功能影响的机制。方法选择成年Wistar大鼠20只,10只为假手术组,10只为模型组(去卵巢组),6周后处死大鼠,分离摘取肾上腺,称重后常规石蜡包埋切片,分别进行ERα、ERβ抗体的免疫组织化学染色,并取动物血清测量雌二醇(E2)和促卵泡生成素(FSH)的含量。结果1.ERα与ERβ在大鼠肾上腺均有表达,主要分布在肾上腺皮质部,以球状带和束状带为主,网状带有少量分布,ERβ的表达强于ERα。2.ERβ在大鼠模型组的表达强于假手术组,有统计学意义,ERα在模型组的表达略强于假手术组,但无统计学意义。3.卵巢切除后,大鼠血清FSH升高,E2下降,与假手术组相比,有显著差异。结论卵巢切除可影响雌激素受体在大鼠肾上腺的表达,尤其是对ERβ表达的影响较为显著。卵巢切除能够减少大鼠血清雌激素的分泌,使垂体反馈性的增加FSH的分泌。Objective The mechanism of the influence on adrenal glands of the ovariectomy rats was studied by observing the expression of estrogen receptor subtype in adrenal glands of the rats. Methods Twenty adult female Wistar rats were divided into 2 groups：the sham-operated group（10 rats） and the model group（10 rats）.After 6 weeks all rats were killed and the adrenal glands were excised,and the tissues in each group were studied with the method of immunohistochemistry.The level of estrogen（E2） and follicle stimulating hormone（FSH） in blood serum was measured by radiation immunohistology. Results 1.Staining for ERα and ERβ were the densest in zona fasciculats and zona reticularis with lesser staining in the zona glomerulosa.ERα expressed weaker than ERβ.2.ERβ expressed in the sham-operated groups and less intensity than in the model groups,it had statistically significant.3.The level of FSH in the serum of the ovariectomy rats was increasing and the E2 was decreasing,there was great difference between the sham-operated groups and the model group.Conclusion The expression of either ERα or ERβ on the rat adrenal gland was obviously influenced by ovariectomy,especially the expression of ERβ.Ovariectomy could decrease E2 in serum and increase FSH which excretes from pituitary through feedback.

关 键 词：卵巢切除 肾上腺 雌激素受体 免疫组织化学 大鼠 

分 类 号：R332[医药卫生—人体生理学]