槟榔提取液对血管内皮细胞分泌内皮素1的影响  

Effects of aqueous areca nut extract on the level of endothelin-1 secreted by endothelial cells.

作　　者：庞丹琳[1] 彭解英[1] 尹晓敏[1] 吴颖芳[1] 李明 

机构地区：[1]中南大学湘雅医院口腔科,湖南长沙410008 [2]长沙市口腔医院

出　　处：《临床口腔医学杂志》2008年第2期83-85,共3页Journal of Clinical Stomatology

基　　金：湖南省科技厅社会发展基金资助(02SSY3052)

摘　　要：目的:观察在不同浓度槟榔提取液(aqueous areca nut extract,AANE)作用下血管内皮细胞(endothelial cell,EC)分泌内皮素1(endothelin-1,ET-1)的变化,探讨EC及ET-1在口腔黏膜下纤维性变(oral submucous fibrosis,OSF)发生发展中的作用。方法:体外培养人脐静脉内皮细胞,换入含终浓度分别为0、50、100、200、400、600、800μg/mlAANE的无血清DMEM培养基,h后用放射免疫法检测细胞培养上清液中ET-1的水平。结果:100μg/ml48及以上浓度的AANE作用后,分泌ET-1的量较未加AANE组显著增加,并呈浓度依赖性。结论:一定浓度的ECAANE能使EC异常分泌ET-1,可能参与OSF病理过程的发生发展。Objective：To assess the possibility of endothelial cells （EC）and endothelin-1（ET-1）in the process of oral submucous fibrosis（OSF） by observing the direct effect of aqueous areca nut extract （AANE） on the level of ET-1 secreted by EC. Method：EC （from ECV-304） were cultured in vitro. EC were incubated together with AANE of different concentration： 0、50、100、200、400、600、800 μg/ml, and after 48 hours we investigated the level of ET-1. Result：The level of ET-1 secreted by EC was increased when EC were affected by AANE of concentration at 100 μg/ml or more for 48 hours, and positive correlations were found between intensity of increasing agents and the dosage.Conclusion：AANE of some concentration could play an important role in the process of OSF through making the content of ET-1 increase by injuring EC.

关 键 词：槟榔提取液 口腔黏膜下纤维性变 血管内皮细胞 内皮素 

分 类 号：R780.2[医药卫生—口腔医学] R781.5[医药卫生—临床医学]