ISSR标记对34份樱桃种质资源的遗传分析  

Genetic Diversity and Relationship of Cherry Germplasm by ISSR Markers

作　　者：艾呈祥[1] 张力思[1] 李国田[1] 魏海蓉[1] 樊靖[2] 刘庆忠[1] 

机构地区：[1]山东省果树研究所,山东省果树生物技术育种重点实验室,泰安271000 [2]西南林学院园林学院,昆明650224

出　　处：《中国农学通报》2008年第4期47-51,共5页Chinese Agricultural Science Bulletin

基　　金：山东省农业科学院青年基金项目"甜樱桃自交不亲和QTL定位与分析";科技部国际合作项目"早熟;大果甜樱桃分子标记育种"(2006DFA33130);国家‘863’计划项目"优质多抗果树分子育种技术与品种创制"(2006AA100108-4-12-9)

摘　　要：利用ISSR标记对34份樱桃种质资源进行遗传多样性检测。结果发现,ISSR标记能够揭示材料间较高的遗传多样性。每个引物可获得6～12条DNA片段,平均为8.76条;17个ISSR引物共扩增出149条DNA片段,其中143条具有多态性,多态性比率(PPB)为95.97%;平均多态信息量(PIC)为0.90;每个位点有效等位基因数(Ne)为1.914;材料间遗传相似系数GS变幅为0.44～0.91,平均达0.73。通过聚类,从分子水平对樱桃种质资源的遗传关系进行分析,并对34份资源进行分类,ISSR标记能将34份樱桃种质完全区分开,为樱桃种质资源的研究利用提供参考。The genetic diversity and genetic relationships among 34 cherry accessions, including 3 interspecific progenies, 1 Prunus pseudocerasus, and 30 Prunus avium, were evaluated by ISSR markers. In ISSR analysis, a total of 149 bands were detected, among which 143 bands were polymorphic. The number of bands from each ISSR marker ranged from 6 to 12, with an average of 8.76. The PPB, PIC and Ne were 95.97%, 0.90 and 1.914, respectively. The ISSR-derived genetic similarity （ISSR-GS） ranged from 0.44 to 0.91, with a mean of 0.73. The cluster analysis indicated that all the 34 sweet cherry germplasm could be distinguished by ISSR markers. The genetic relationship cherry accessions were analyzed and 34 germplasm were classified on molecular level by clustered analysis, which provided reference for research in cherry germplasm.

关 键 词：樱桃 ISSR 遗传多样性 聚类分析 

分 类 号：S662.5[农业科学—果树学]