野生种多毛、醋栗番茄与栽培种番茄的指纹图谱分析  

Fingerpringting Analysis of L.hirsutum and L.pimpinellifolium Wild Species and Cultivated Tomato

作　　者：吴玉辉[1] 杨建平[1] 李君明[2] 刘磊[2] 

机构地区：[1]山东农业大学园艺科学与工程学院,泰安271018 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《中国农学通报》2008年第4期80-84,共5页Chinese Agricultural Science Bulletin

基　　金：北京市自然科学基金(6042022)

摘　　要：利用CAPS和SSR两种分子标记技术,对野生种多毛和醋栗番茄与栽培种番茄的DNA指纹图谱进行了分析。结果表明,30对CAPS和10对SSR引物中,分别有19对和7对引物产生清晰明显且稳定的多态性条带。利用两种标记共获得了98个标记位点,分析表明SSR标记的检测效率高于CAPS标记。相对于栽培种而言,多毛番茄有16.3%的特异信息,醋栗番茄有10%的特异信息。该些标记为番茄遗传图谱的构建、重要基因的标记及遗传育种提供了有力工具。Using CAPS and SSR two molecular techniques, the fingerprinting of wild tomato and cultivated tomato were analyzed. In this study, 19 out of 30 pairs of CAPS primers and 7 out of 10 SSR primers were selected, which gave stable and polymorphic amplification profiles. This two molecular techniques produced 98 bands, the results showed that the detectability of SSR is better than CAPS, 16.3 percent were form L.hirsutum, 10 percent were from L.pimpinellifolium, this two wild tomatoes could be used in genetics and breeding of tomato availably.

关 键 词：野生番茄 栽培番茄 指纹图谱 CAPS SSR 

分 类 号：S58[农业科学—作物学]