一个水稻小G蛋白OsRan1的克隆及表达分析  

Isolation and Characterization of a Rice Gene OsRan1 Coding a Small G Protein

作　　者：席江[1] 杜志如[1] 徐永菊[1] 张琦[1] 万佳[1] 江斌[1] 徐正君[1] 

机构地区：[1]四川农业大学水稻研究所,温江611130

出　　处：《中国农学通报》2008年第4期109-114,共6页Chinese Agricultural Science Bulletin

基　　金：教育部长江学者和创新团队发展计划资助项目(IRT0453);四川省青年科技基金资助项目"水稻逆境反应基因克隆与分析"

摘　　要：逆境对于水稻产量和品质有很大影响,研究在逆境下水稻基因的表达变化对于研究水稻抗逆有着重要意义。运用荧光差异显示(Fluorescence Differential Display,FDD)技术,从低温处理的水稻中克隆到OsRAN1基因并通过半定量PT-PCR分析该基因在低温和高盐逆境下的表达。该基因cDNA序列全长为984bp,含有一个编码221个氨基酸残基的开放阅读框,推测分子量为25.0KD,此氨基酸序列具有GTPase活性区域,属于小G蛋白Ran亚家族,通过氨基酸相似性比对发现,Ran家族氨基酸序列高度保守,OsRAN1与TaRAN1和AtRAN1相似性分别达到98.19%和92.76%。通过半定量PT-PCR分析,发现在低温胁迫下,OsRAN1在根和叶鞘中表达量均有增加,尤其是高盐胁迫下根中OsRAN1表达量有迅速升高然后降低的变化。推测该基因在水稻逆境应答中起着重要作用。The disadvantage circumstance is an important factor in impacting on the yield and quality of rice The expression analysis of gene under stress helps a lot in studying of stress resistance of rice. A rice gene, OsRAN1, was isolated from rice treated with cold-stress by the fluorescence differential display （FDD） screening method,and its expression pattern was analyzed by simi-quantitative RT-PCR analysis under cold and salt stress. Its cDNA sequence （984bp） contains an ORF encoding a 221 amino acids protein （25.0KD）,and the protein belongs to Ran subfamily of the small G protein, for containing the typical GTPase structure. At the amino-acid sequence level, OsRAN1 exhibit high homology to TaRAN1 and AtRAN1 （98.19% and 92.76%）. By simi-quantitative RT-PCR analysis, the expression of OsRAN1 at the transcription level was increased under the low temperature. Especially under high salt condition, the expression level in root shows a process of increase, and then decrease. We suggest the OsRAN1 gene could play an important role of stress response in rice.

关 键 词：水稻 RAN 小G蛋白 荧光差异显示 克隆 表达 

分 类 号：Q786[生物学—分子生物学]