水稻核糖体蛋白(OsRPL14)基因的克隆及表达分析  

Cloning and Expression Analysis of a Ribosomal Protein from Rice(OsRPL14)

作　　者：杜志如[1] 席江[1] 万佳[1] 江斌[1] 徐永菊[1] 张琦[1] 徐正君[1] 

机构地区：[1]四川农业大学水稻研究所,温江611130

出　　处：《中国农学通报》2008年第4期130-134,共5页Chinese Agricultural Science Bulletin

摘　　要：通过荧光差异显示(fluorescent differential display,FDD)以及RT-PCR技术,得到一个水稻核糖体蛋白基因。该基因的cDNA全长为565bp,编码一个具有134个氨基酸残基的多肽,推测分子量是15.4kD。与其他植物中核糖体蛋白L14的相似性为82.84%～88.06%,命名该基因为OsRPL14。在低温(8℃)及干旱条件处理下,该基因表达量在根和茎中都有比较明显的增加趋势。推测该基因在逆境反应中起着重要作用。Using a fluorescent differential display method, a gene was isolated from rice seedling. The full length cDNA was 565 bp and encoded a 134 amino acid（15.4kD）. The results of similar analysis indicated that OsRPL14 had 82.84%-88.06% identities with other ribosomal proteins, named OsRPL14. The level of the gene expression was enhanced in response to cold（8℃）and dry. We suggest the gene might play important roles in stress respones.

关 键 词：水稻 核糖体蛋白 克隆 表达 

分 类 号：Q78[生物学—分子生物学]