苹果轮纹病菌拮抗放线菌的筛选与鉴定  

Selection and Identification of the Antagonistic Actinomycetes Against Apple Ring Rot Disease

作　　者：展丽然[1] 张克诚[1] 冉隆贤[2] 石义萍[1] 阿拉坦夫[1] 王万群[1] 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100094 [2]河北农业大学林学院,保定071001

出　　处：《中国农学通报》2008年第4期341-344,共4页Chinese Agricultural Science Bulletin

基　　金：科技部农业科技成果转化项目"生物农药武夷菌素生产技术改进与产业化"(05EFN216900354);国家科技支撑计划"重大病虫害生物防治新技术"(2006BAD08A02)

摘　　要：为了寻找防治苹果轮纹病的生防放线菌,该文采用平板对峙法、管碟法相结合的方法对供试菌株进行筛选,初筛数据表明,供试菌株对苹果轮纹病菌均有不同程度的抑制作用,其中S-20、Z-6、Z-8、Z-23、Z-42和5-1的抑菌率在50.0%以上,占试验总菌株的66.7%,其中5-1抑菌率达72.0%。管碟法进行发酵液拮抗活性测定,发现5-1抑菌带最宽,直径达24.9mm。效果最显著。为了加深对该菌株的了解,此文对其形态特征、培养特征及生理生化特征进行了测定,初步鉴定为链霉菌属(Streptomyces spp.)的白孢类群(Albosporus)。最后,抑菌谱试验表明,5-1不仅对苹果轮纹病菌有抑制作用,对其他多种病原真菌作用同样显著,抑菌谱广,有可能为开发新型生物农药提供可能。In order to find actinomycs against physalospora piricola for biological control. The provided strains were screened through pair-culture and cylinder-plate method. The results showed that the inhibition rate of S-20, Z-6,Z-8,Z-23,Z-42,5-1 are more than 50.0%. In which the 5-1 accounted for 72.0%. In the tests of the metabolic products in fermentation liquid, The inhibitive zone is 24.9mm. According to the morphological, physiological and biochemical characteristics, 5-1 was identified as Albosporus of streptomyces. Inhibitive range showed the inhibitive range of 5-1 obroadly. Not only has effect on physalospora piricola, but also on other pathogen, It provide possibility to exploit new biological pesticides.

关 键 词：苹果轮纹病 分离 筛选 鉴定 

分 类 号：S476.8[农业科学—农业昆虫与害虫防治]