HPLC-ELSD测定复方丹参片中三七皂苷R_1、人参皂苷Rg_1、人参皂苷Rb_1的含量  

Determiation the Contents of Notoginsenoside R_1,Ginsenoside Rg_1 and Rb_1 in Compound Dan shen Tablets by EIPLC-ELSD

作　　者：黄海欣 高光伟[2] 

机构地区：[1]南阳市药品检验所,河南南阳473061 [2]河南省南阳市中心医院

出　　处：《中国药师》2008年第4期404-406,共3页China Pharmacist

摘　　要：目的:建立复方丹参片中三七皂苷R_1,人参皂苷Rg_1及人参皂苷Rb_1含量的方法。方法:采用HPLC-ELSD方法,Agilent ODS C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,ELSD为栓测器,载气为氮气流速2.0 L·min^(-1),漂移管温度70℃。结果:三七中皂苷R_1,人参皂苷Rg_1及人参皂苷Rb_1的线性范围分别为:0.21～2.14μg(r=0.9998),1.0～10.0μg(r=0.999 3)和0.93～9.28μg(r=0.9991),平均回收率(n=5)分别105.7%(RSD 2.0%),100.7%(RSD 1.2%)和101.4%(RSD1.2%)。结论:方法简单,快速和准确,用于复方丹参片的质量控制。Objective： To establish a method for determination the contents of notoginsenoside R1, ginsenoside Rg1 and Rb1 in compound danshen tablets. Method： HPLC-ELSD was used,the separation was performed on Agilent ODS C18 column（250 mm × 4.6 mm,5 μm） with acetonitrile-water as mobile phase, the column temperature was 35 % ,gradient elution, an ELSD was used as detector with Nitrogen gas flow rate of 2.0 L·min^- 1 and drift tube temperature of 70℃. Result： The linear ranges of Notoginsenoside R1 ginsenoside Rg1, ginsenoside Rb1 ,were at 0.21 - 2.14 μg（ r = 0.999 8 ）, 1.0 - 10.0 μg（ r = 0.999 3 ） and 0.93 - 9.28 μg （ r = 0.999 1 ） respectively,the average recoveries（n= 5 ） were 105.7% （RSD 2.0% ）., 100.7% （RSD 1.2% ） and 101.4% （RSD 1.2% ） respectively. Conclusion： The method is simple, rapid and accurate, it can be used for quality control of compound danshen tablets.

关 键 词：复方丹参片 三七皂苷R1 人参皂苷Rg1 人参皂苷Rb1 高效液相色谱-蒸发光散射检测器 

分 类 号：R927.2[医药卫生—药学]