1,8-二羟基蒽醌修饰的钌配合物的合成及其与DNA相互作用的研究  

Studies on the interaction of a novel ruthenium(II) complex hybrid by 1,8-dihyroxyl-9,10-anthraquinone and calf-thymus DNA

作　　者：梅文杰[1] 黄东纬[1] 王娜[1] 刘云军[1] 王定勇[1] 梁宝霞[1] 

机构地区：[1]广东药学院药科学院,广东广州510006

出　　处：《广东药学院学报》2008年第1期54-57,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省自然科学基金博士启动基金项目(04300624);广东省科技计划项目(2007B031513004)

摘　　要：目的用1,8-二羟基-9,10-蒽醌-醛-3与cis-Ru(bpy)2Cl2.2H2O为原料合成钌多吡啶配合物[Ru(bpy)2HAIP]2+(HAIP=(1,8-二羟基-9,10-蒽醌)咪唑[4,5-f][1,10]菲咯啉)(1),并对其与DNA的识别机理进行初步研究。方法采用元素分析、电喷雾质谱以及电子吸收光谱对目标化合物进行了表征,并采用电子吸收光谱、荧光发射光谱及热变性实验研究配合物与小牛胸腺DNA的识别作用。结果电子吸收光谱研究表明,当[DNA]/[Ru]=0.1,钌配合物1的特征MLCT(metal to ligandcharge transfer)荷移跃迁和IL(intraligand charge transfer)荷移跃迁的减色率分别为9%(Δλ=2 nm)和15%(Δλ=1 nm),根据EB-荧光淬灭实验结果计算得到了钌配合物1与DNA相互作用的表观结合常数约为5.13×104,热变性实验结果表明在钌配合物1存在下,小牛胸腺DNA的熔点温度升高约4.8℃,结论目标化合物1可能以非经典的插入模式与DNA分子结合。A novel ruthenium complex [ Ru（bpy） 2HAIP] （ bpy = bipyridine; HAIP = （ 1,8-dihydroxy-9,10-anthraquinone） imidazo [4,5-f] [ 1,10 ] phenanthroline）, 1, has been synthesized and characterized by elementary analyzer, ESI-MS, as well as spectroscopy methods. The DNA-binding properties of this complex with calf thymus DNA have been studied by utilizing electronic spectra and emission spectrA. The results of thermal denaturation of DNA show that complex 1 may bind to DNA in a non-classic intercalating binding mode, and the electronic titration experiment show that at the ratio [ DNA ]/[ Ru ] = 0.1 ,the hypochromism for IL transition and MLCT transition of ruthenium （Ⅱ） complex 1 is 15 % （ △λ = 1 nm） , and 9% （ Ak = 2 nm）, respectively, and the appearance binding constant calculated for complex 1 according to the decay of emission spectra is 5.13 ~ 10^4.

关 键 词：钌(Ⅱ)配合物 芦荟大黄素苷 小牛胸腺DNA 插入作用 

分 类 号：O641.4[理学—物理化学]