黄芪对梗阻性黄疸幼鼠T淋巴细胞免疫功能的影响  

Effect of Astragalus on T Lymphocyte Immune Function in Immature Rats with Obstructive Jaundice

作　　者：韩兆东[1] 阮月芹[1] 张兰[1] 樊其忠[1] 傅廷亮[1] 

机构地区：[1]滨州医学院附属医院,滨州256603

出　　处：《国际中医中药杂志》2008年第2期87-89,共3页International Journal of Traditional Chinese Medicine

基　　金：山东省教育厅科技发展计划项目（编号：J05L11）

摘　　要：目的探讨黄芪对梗阻性黄疸幼鼠血液和肠黏膜T淋巴细胞免疫功能的影响。方法以3周龄Wistar幼鼠为载体，随机分为4组：正常对照组、假手术组、梗阻性黄疸（OJ）组、OJ＋黄芪组（OJ＋A），每组各12只。利用流式细胞仪检测实验幼鼠外周血及肠黏膜T淋巴细胞（CD4、CD8）的百分率，计算CIM，CD8比值。结果OJ组外周血和肠黏膜CD4细胞、CD4／CD8比值明显低于正常对照组及假手术组，有显著性差异（P〈0．05）。OJ＋A组外周血和肠黏膜CD4细胞、CD4／CD8比值均较OJ组升高，有显著性差异（P〈0．05）。结论黄芪能提高OJ幼鼠T淋巴细胞的免疫功能。Objective To evaluate the effect of Astragalus on T lymphocyte immune function in peripheral blood and intestinal mucosa of immature rats with obsauctive jaundice. Methods Three-week-old Wistar rats were randomly divided into four groups （n= 12, in each）： control group, sham operation group, obstructive jaundice （OJ） group, OJ＋ Astragalus （OJ＋A） group. The percentage of CD4 and CD8 T lymphocytes, the ratio of CD4 to CD8 in peripheral blood and intestinal mucosa of immature rats was detected by flow cytometry. Result The percentage of CD4^＆＋ cell and the ratio of CD4 to CD8 in the OJ group were lower than those in the control group and the sham operation group, with significant difference （ P〈 0.05）. The percentage of CD4 cell and the ratio of CD4 to CD8 in the OJ＋A group were higher than those in the OJ group （P〈 0.05）. Conclusion Astragalus can increase T lymphocyte immune function in OJ immature rats.

关 键 词：黄芪 梗阻性黄疸 细胞免疫 幼鼠 

分 类 号：R28[医药卫生—中药学]