穿心莲内酯对肝硬化大鼠肠道通透性的作用及临床意义  

Role of andrographolide in the permeability of intestinal tract of cirrhosis rats and its clinical significance

作　　者：覃慧敏[1] 吴春明[2] 李洪涛[3] 宋建新[3] 

机构地区：[1]三峡大学第一临床医学院宜昌市中心人民医院感染科,湖北省宜昌市443003 [2]温州市中医院肝病科,浙江省温州市325000 [3]华中科技大学同济医学院附属同济医院感染科,湖北省武汉市430030

出　　处：《世界华人消化杂志》2008年第8期839-843,共5页World Chinese Journal of Digestology

基　　金：湖北省卫生厅科研基金;No.NX200506~~

摘　　要：目的:检测穿心莲内酯对肝硬化大鼠肠道通透性的影响,并探讨其可能的临床意义.方法:采用高效液相色谱法(high perfomance liquid chromatography,HPLC)检测研究对象尿液标本中甘露醇(mannitol,M)和乳果糖(lactulose,L)排出率比值,通过计算甘露醇和乳果糖的排出率来评估肠道通透性.结果:在上述色谱条件下,肝硬化大鼠尿液标本中乳果糖/甘露醇排出率较正常大鼠(0.036±0.012 vs 0.026±0.009,P<0.05)显著增高.穿心莲及诺氟沙星干预组较对照组L/M显著降低(0.022±0.010,0.022±0.007 vs 0.036±0.012,P<0.05).结论:肝硬化大鼠较正常大鼠肠道通透性显著性增高.肝硬化大鼠中,穿心莲及诺氟沙星干预组大鼠的肠道通透性较对照组显著降低.AIM： To detect the impact of andrographolide on the permeability of intestinal tract of cirrhosis rats by high performance liquid chromatography and to probe its clinical significance. METHODS： The discharge rate of mannitol （M） and lactulose （L） in cirrhosis rats was detected by high performance liquid chromatography （HPLC） and compared with the standard curve. RESULTS： The lactulose/mannitol （L/M） discharge rate in cirrhosis rats was significantly higher than that in normal rats （0.036 ± 0.012 vs 0.026 ± 0.009, P 〈 0.05） and significantly lower in the andrographolide and norfloxacin intervention group than that in the control group （0.022 ±0.010, 0.022 ±0.007 vs 0.036 ± 0.012, P 〈 0.05）. CONCLUSION： The permeability of intestinal tract of cirrhosis rats is significantly higher than that of normal rats and significantly lower in the andrographitis and norfloxacin intervention group than that in the control group.

关 键 词：穿心莲内酯 肝硬化 肠道通透性 高效液相色谱法 

分 类 号：R285.5[医药卫生—中药学]