乌梅丸对溃疡性结肠炎大鼠结肠组织NF-κB p65的影响  

Effect of Wumeiwan on NF-κB p65 in colon tissue of rats with ulcerative colitis

作　　者：范恒[1] 段雪云[2] 庄雄[1] 吕建芳[1] 寿折星[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院中西医结合科,湖北省武汉市430022 [2]湖北中医学院附属医院药剂科,湖北省武汉市430061

出　　处：《世界华人消化杂志》2008年第8期896-899,共4页World Chinese Journal of Digestology

基　　金：中国博士后科学基金;No.2005037679~~

摘　　要：目的:比较乌梅丸与西药柳氮磺胺吡啶SASP组对溃疡性结肠炎大鼠结肠组织NF-κB p65疗效的大小,并分析其作用机制.方法:应用2,4.二硝基氯苯(DNCB)免疫加醋酸局部灌肠法建立UC大鼠模型,将56只健康SD大鼠(雌雄各半),按雌雄随机分4组,分别为乌梅丸组、SASP组、模型组和正常组,分别观察治疗后大鼠结肠黏膜组织匀浆NF-κB p65的变化.结果:在正常结肠组织中NF-κB p65无或仅有弱阳性表达,模型组结肠黏膜组织NF-κB p65阳性细胞表达率明显高于正常组(45.67%±4.25%vs 10.45%±5.20%,P<0.05),乌梅丸(26.32%±9.65%)和SASP(31.23%±5.18%)组阳性细胞率则明显低于模型组,乌梅丸组阳性细胞率较SASP组更低.结论:NF-κB与UC发病关系密切,乌梅丸可能通过抑制NF-κB p65活性调节免疫功能达到治疗的目的.AIM： To compare the effects of Wumeiwan （WMW） and solfasalazine （SASP） on NF-κB p65 in colon tissue of rats with ulcerative colitis （UC） and to analyze their mechanism of action. METHODS： A model of UC was induced by administration of dinitro-chlorobenzene （DNCB） and acetic acid. Fifty-six SD rats （28 males and 28 females） were randomly divided into normal control group （n = 14）, model group （n = 14）, SASP group （n = 14）, and WMW group （n = 14）. Changes of NF-κB p65 in rat colon tissue were observed after treatment with DNCB and acetic acid. RESULTS： NF-κB p65 was not expressed or weakly expressed in normal colon tissue. The positive expression rate of NF-κB p65 was significantly higher in WMW and SASP groups than in normal control group（26.32% ± 9.65%, 31.23% ± 5.18% vs 45.67% ±4.2%, P 〈 0.01）. The positive expression rate of NF-κB p65 was significantly lower in WMW group than in model group. There were significant differences between WMW and SASP groups （P 〈 0.05）. CONCLUSION： NF-κB is closely related with the development of UC. WMW can treat UC by suppressing the activity of NF-κB p65.

关 键 词：溃疡性结肠炎 溃疡性结肠炎 NF—κB P65 乌梅丸 

分 类 号：R285.5[医药卫生—中药学]