不同纯化方法处理DNA合成引物对PCR扩增效率的影响  

The Influence of the Synthesized DNA Primers Purified with Different Methods on PCR Amplification Efficiency

作　　者：赵庆萱[1] 石乐琴[1] 王春晖[1] 

机构地区：[1]卫生部兰州生物制品研究所

出　　处：《微生物学免疫学进展》1997年第3期65-67,共3页Progress In Microbiology and Immunology

摘　　要：对ＤＮＡ合成的幽门螺杆菌尿素酶引物ＨＰ１、ＨＰ２、ＨＰ３、ＨＰ４进行了几种不同的纯化试验，分别采用无水乙醇沉淀法、ＮＴ柱及聚丙烯酰胺凝胶电泳方法，对其相应的纯化收率，ＰＣＲ扩增效率作了比较及分析。琼脂糖凝胶电泳结果证实，以无水乙醇沉淀纯化方法的ＰＣＲ扩增效果较为理想。该方法操作简便、稳定高效、省时省力、成本低。为此建议用该法处理ＤＮＡ合成引物。The synthesized DNA primers HP 1 HP 2 HP 3 and HP 4 were purified with different methods:ethanol precipitation,NT column and polyacrylamide gel electrophoresis.The recovery rate of purification and the efficiency of PCR of these primers were analyzed respectively.The result of agarose gel electrophoresis showed that the method of ethanol precipitation is better than others.This method has the advantage of simplication,efficiency,stability,time saved and low cost.We commend this method on purifing synthesized DNA primers so as to attain satisfactory result.

关 键 词：DNA 合成引物 纯化 收率 聚合物酶链反应 

分 类 号：Q523.03[生物学—生物化学]