分泌型重组人粒细胞-巨噬细胞集落刺激因子的纯化  

Purification of Secretory Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF)

作　　者：林殿海[1] 杨桂云[1] 赵立欣 刘淑玲[1] 迟春萍[1] 刘东梅 李建平[1] 张秀霞[1] 金铎[1] 张春力 

机构地区：[1]卫生部长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》1997年第3期132-135,共4页Chinese Journal of Biologicals

摘　　要：GM－CSF工程菌W3100经发酵培养后，将菌体低渗裂解，经流水柱─分子筛─阴离子交换树脂的一系列层析，最后得到纯化的GM－CSF，其纯度为98％以上，比活性大于20×107。After genetically engineered E. coli W3100/pGM-CSF was cultured in fermenter, cells were lysated by osmotic-shock method. GM-CSF was purified by hydrophobic,molecular sieze and ion exchange charomotagraphy. The purity and specific activity of theGM-CSF reached >98% and >2.0×107 respectively.

关 键 词：GM-CSF 纯化 粒细胞巨噬细胞 集落刺激因子 

分 类 号：R392－33[医药卫生—免疫学]