抗菌肽CM4抗K562癌细胞的超微结构研究  

Ultrastructure Observation of K562 Leukemia Cells Treated with Antibacterial Peptide CM4 Component.

作　　者：张双全[1] 贾红武[1] 戴祝英[1] 

机构地区：[1]南京师范大学生物系,南京210097

出　　处：《生物化学与生物物理进展》1997年第2期159-163,共5页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目 ;国家教育委员会留学回国人员资助费支持项目

摘　　要：报道了家蚕抗菌肽CM4抗K562癌细胞的体外实验研究 .结果表明 :纯化后的家蚕抗菌肽CM4对培养的K562 (人髓样白血病细胞 )有很强的杀伤作用 .用扫描和透射电镜观察超微结构以及用激光共聚集显微断层图像分析 ,表明微量纯化的抗菌肽CM4能使K562癌细胞产生一系列的病理变化 ,可造成细胞高度肿胀 ,膜与胞质分离 ,细胞器和膜结构排列紊乱 ,细胞表面微绒毛消失 ,出现不规则的孔洞 ,细胞骨架严重破坏 ,膜局部结构破裂 ,缺损 ,胞浆内容物大量外泄 ,最终细胞解体 。The effect of antibacterial peptide CM4 component act on the K562 cancer cells in vitro is reported. The tests showed that: the purified antibacterial peptide CM4 component can kill human myelogenous leukemia cancer (K562). The observation of ultrastructure of the cancer treated with the antibacterial peptides by using the scanning and transmission electron microscope and the microfault picture analyses of laser confocal microscope. The results showed that the pruified antibacterial peptides brought about a series of pathological changes on cancer cells. As a result, the cells were high swelling, plasmolysis and disorders in subcellular and membrane structrue occurred, outer coat of cell dissolved, thus appearing irregular holes opening in a utensil and serious broken of skeleton structure of the cells. The membrane structure of some areas was broken and injured and intracellular substances leaked out. As a result, the cells disintegrated into small fragmants and died.

关 键 词：抗菌肽 癌细胞 超微结构 肿瘤 

分 类 号：R977.6[医药卫生—药品] R730.53[医药卫生—药学]