载脂蛋白AⅡ保护内皮细胞的实验研究  

The Protective Effects of Apolipoprotein AⅡ on Endothelial Cell Injured by Low Density Lipoprotein in vitro

作　　者：黎健[1] 蒋雷[1] 刘清华[1] 

机构地区：[1]卫生部北京老年医学研究所,北京100730

出　　处：《生物化学与生物物理进展》1997年第4期344-347,共4页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助课题! (39170 789)

摘　　要：以细胞形态观察、细胞存活率、乳酸脱氢酶释放和前列环素测定等方面研究载脂蛋白AⅡ保护内皮细胞的作用 .结果显示 ,载脂蛋白AⅡ可部分拮抗低密度脂蛋白对内皮细胞的损伤作用 ,维持内皮细胞形态基本正常 ,乳酸脱氢酶释放减少 。To study the protective effects of apolipoprotein AⅡ (apoAⅡ) on endothelial cells injured by low density lipoprotein (LDL) in vitro, human umbilical vein endothelial cells were cultured and divided into four groups: control, HDL+LDL, apoAⅡ+LDL and LDL group, which were observed the morphological changes with phase-contrast and transmission electron microscope and measured the release of lacate dehydrogenase (LDH) and level of 6-keto-prostaglandin F1α (PGF1α). The endothelial cells injured by LDL showed cell contraction, increased release of LDH and decreased level of 6-keto-PGF1α. However, normal morphology and LDH release as well as PGI 2 synthesis in endothelial cells were found when HDL or apoAⅡ was added to culture media before LDL injury. The results indicated that both HDL and apoAⅡ could resist the injurious effect of LDL on cultured endothelial cells.

关 键 词：动脉粥样硬化 内皮细胞 载脂蛋白AⅡ 

分 类 号：R543.502[医药卫生—心血管疾病]