用Blue Sepharose CL-6B快速纯化天花粉蛋白  

Rapid Purification of Trichosanthin by Blue Sepharose CL-6B

作　　者：袁惠东[1] 夏其昌[1] 张祖传[1] 

机构地区：[1]中国科学院上海生物化学研究所,上海200031

出　　处：《生物化学与生物物理进展》1997年第4期369-373,共5页Progress In Biochemistry and Biophysics

摘　　要：差光谱显示染料cibacronblueF3GA与天花粉蛋白 (TCS)有特异性结合 ,复合物在可见光部分的最大吸收波长在 690nm ,摩尔消光系数ε =2 6× 1 0 - 3 (mol/L) - 1·cm- 1,解离常数Kd =1 8μmol/L,0 5mol/LNaCl可使复合物解离 .根据这一特点 ,用Blue SepharoseCL 6B凝胶从栝篓块茎中亲和纯化了TCS .此法快速、简便、高效 ,易于大量制备 .It is revealed by difference spectra that cibacron blue F3GA could bind specifically to Trichosanthin (TCS). Thespectralabsorption maximum is on 690nm with molar absorption coefficient of 26×10 -3 (mol/L) -1·cm -1. The dissociation constant is 18 μmol/L, and 05 mol/L NaCl could dissociate the complex. Based on these results, TCS is purified from the root tuber of Trichosanthes Kirilowii by Blue Sepharose CL-6B. This method is rapid, simple, efficient and could be applied in mass preparation of TCS.

关 键 词：天花粉蛋白 Cibacronblue FEGA 亲和层析 纯化 

分 类 号：Q946.1[生物学—植物学]