核酸探针与酶免疫反应板定向结合的方法  

A Method for Site-specific Conjugation of Oligonucleotide Probe on ELISA Plate

作　　者：刘吉荣[1] 吴婉芳[1] 秦雨春[1] 张玉[1] 马官福[1] 

机构地区：[1]首都儿科研究所,北京100020

出　　处：《生物化学与生物物理进展》1997年第4期379-380,共2页Progress In Biochemistry and Biophysics

摘　　要：为了制备一种可供寡核苷酸探针定向结合的酶免疫反应板 ,利用部分水解的尼龙包被酶免疫反应板 ,通过尼龙所含的氨基基团与寡核苷酸探针所含的 5′磷酸基团 ,在水溶性碳二亚胺的催化下 ,与酶免疫反应板发生 5′端特异性定向共价结合 ,并对肺炎支原体的PCR扩增产物进行了重复检测 ,结果重复性好 ,方法稳定 .利用该方法制备的酶免疫反应板可用于核酸探针的临床检测 .In order to prepare an ELISA plate which can be used for site-specific conjugation of oligonucleotide probe, partially hydrolysed nylon was used to coat ELISA plate, oligonucleotide probes were conjugated to ELISA plate by covalent conjugation of 5′ phosphate group of the probe with the amino groups of the hydrolysed nylon under the catalysis of water soluble carbodiimide. The method was used to detect PCR product of Mycoplasma pneumoniae, and reproducible result was acquired. The reported method can be used in molecular detection of clinical pathogens.

关 键 词：寡核苷酸探针 酶免疫反应板 临床检验 

分 类 号：R446.1[医药卫生—诊断学]