应用流式细胞仪检测原因未明“血小板病”患者血小板膜糖蛋白的改变  

Study on Platelet Membrane Glycoproteins in Obscure so Called Thrombopathy by Flow Cytometry

作　　者：杨林花[1] 沈迪[2] 王爱莲[2] 魏文宁[2] 张玉金[2] 刘仲萍[2] 杨锐[2] 杨焰 

机构地区：[1]山西医科大学第二医院,太原030001 [2]同济医科大学附属协和医院

出　　处：《中华物理医学杂志》1997年第3期165-167,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金(39370322)

摘　　要：目的:了解原因未明"血小板病"患者血小板膜糖蛋白(GP)的变化。方法:应用流式细胞仪检测了8例原因未明"血小板病"患者血小板膜GPⅠb、GPⅡb、GPⅢa、GPⅠb/Ⅸ和GPⅡb/Ⅲa。结果:发现血小板膜GPⅠb和GPⅠb/Ⅸ复合物降低,平均荧光道数分别为58.5±3.51和62.13±2.17,对照组为65.22±4.49和66.0±4,00,与对照组比较有明显统计学意义(P<0.01和P<0.05)。GPⅢa百分标记率为68.21±3.45,对照组为73.72±4.11(P<0.05)。GPⅡb和GPⅡb/GPⅢa未显示明显异常。结论:血小板膜GP的降低,可能是造成患者出血和血小板聚集功能异常的原因之一。Objective: To estimate the changes of platelet membrane glycoprotcins (GP)in the patients with obscure so called thrombopathy. Methods: Platelct membrane GP Ib, GP Ⅱ b, GP Ⅲ a, GP Ⅰ b/Ⅸ complex and GP Ⅱ b/Ⅲ a complex from eight cases of obscure so called thrombopathy were measured by flow cytometry(FCM). Results: Platelet membrane GP Ⅰ b and GP Ⅰ b/Ⅸ complex were reduced, the mean fluorescence channels were 58.5±3.51 vs 65.22±4.49 and 62.13±2.17 vs 66.0±4.00. The differences were significant as compared with the control group (P<0.01 and P<0.05, respectively). The total percentage of labeled rate of GP Ⅲ a was 68.21±3.45 vs 73.72±4.11(P<0.05). Platelet membrane GP Ⅱ b and GP Ⅱ b/Ⅲ a complex were normal. Conclusion: Decrease of the platclet membrane GP may be one reason of bleeding and platelet aggregation dysfunction of the patients.

关 键 词：血小板病 糖蛋白类 流细胞计数 

分 类 号：R558.02[医药卫生—血液循环系统疾病]