沉默PRL-3基因表达抑制胃癌生长研究  

Role of silencing PRL-3 expression by miRNA interference in the growth of gastric cancer

作　　者：蔡世荣[1] 王昭[1] 陈创奇[1] 崔冀[1] 张常华[1] 何裕隆[1] 詹文华[1] 

机构地区：[1]中山大学附属第一医院胃肠胰腺外科中山大学胃癌诊治中心,广州510080

出　　处：《中华外科杂志》2008年第8期618-621,共4页Chinese Journal of Surgery

基　　金：国家自然科学基金资助项目（30571833）;中国博士后科学基金资助项目（2005038448）

摘　　要：目的探究miRNA干扰沉默PRL-3基因表达对胃癌生长的作用。方法构建表达人工PRL-3 miRNA的慢病毒载体，转染SGC7901细胞并沉默其PRL-3表达；噻唑蓝（MTT）试验评价PRL-3在SGC7901细胞增殖中的作用；移植瘤生长试验评估其在胃癌生长中的作用。结果与转染空载体组比较，转染PRL-3 miRNA的慢病毒载体可抑制SGC7901细胞的增殖。荷瘤动物实验表明，转染PRL-3组肿瘤大小为（1．92±0．18）cm^3，转染空载体组为（4．74±0．39）cm^3，两组差异有统计学意义（P〈0．05）。结论沉默PRL-3表达可抑制胃癌SGC7901细胞的增殖，并抑制移植瘤的生长，可作为一种有潜力的抗肿瘤靶点。Objective To investigate the role of silencing PRL-3 expression by miRNA interference in gastric cancer growth. Methods RNA interference mediated by recombinant lentivirus expressing artficial PRL-3 miRNA was employed to knockdown PRL-3 expression in human SGC7901 gastric cancer cells. MTT assay and tumor implantation experiment were conducted to determine the role of PRL-3 in the proliferation of SGC7901 cells and the tumor growth. Results Transfection of recombinant lentivirus expressing artificial PRL-3 miRNA significantly suppressed the proliferation of SGC7901 cells in vitro. The implanted tumor size of the PRL-3 transfection group was （ 1.92 ± 0. 18） cm^3 , significantly smaller than those in control groups [ （4.74 ± 0. 39） cm^3 ] （ P 〈 0. 05 ）. Conclusions Silencing of PRL-3 significantly suppressed the proliferation of SGC7901 cells and tumor growth in vivo. PRL-3 could be a potential therapeutic target in gastric cancer.

关 键 词：胃肿瘤 PRL-3基因 肿瘤生长 

分 类 号：R686[医药卫生—骨科学]