一种有机磷农药单克隆抗体的制备及其特性研究  

Preparation and characterization of monoclonal antibodies for analysis of organophosphorus pesticides

作　　者：苏婧[1] 薛小平[1] 唐蕊华[1] 谢玉为[1] 尹焕才[1] 

机构地区：[1]西北工业大学生命科学院,陕西西安710072

出　　处：《环境污染与防治》2008年第4期20-22,26,共4页Environmental Pollution & Control

基　　金：陕西农业攻关计划资助项目(No.2006K01-G21)

摘　　要：合成与有机磷农药化学结构相近似的一类半抗原,制备该类半抗原的特异性单克隆抗体,建立酶免疫分析方法,并对4种有机磷农药(毒死蜱、甲基对硫磷、辛硫磷及三唑磷)进行检测。结果显示,此方法对部分有机磷农药灵敏度高,可以简便、快速地检测具有特定结构的有机磷农药,检测灵敏度与被检有机磷农药的化学结构相关。Specific monoclonal antibodies against compounds similar to organophosphorus pesticides, were prepared; the applications in measuring 4 organophosphorus pesticides （chlorpyrifos-ethyl, parathion-methyl, phoxim and triazophos） were demonstrated. A simple and fast detection method for measuring organophosphorus pesticides with high sensitivity and specificity was presented. The structural differences among these pesticides was the major factor affecting the sensitivity of the measurements.

关 键 词：有机磷农药 检测技术 酶免疫分析方法 单克隆抗体 

分 类 号：S481.8[农业科学—农药学]