胰腺发育相关maf基因在胰腺导管和胰岛的表达  

Expression of Pancreatic Development Related maf Genes in Pancreatic Duct and Islet

作　　者：张贤玲[1] 顾燕云[1] 周文中[1] 张宏利[1] 李果[1] 

机构地区：[1]上海市内分泌代谢病研究所,上海交通大学医学院瑞金医院内分泌代谢科,上海市内分泌代谢病临床医学中心,上海200025

出　　处：《生物化学与生物物理进展》2008年第4期431-436,共6页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(30470817)~~

摘　　要：为探讨胰岛功能和发育相关maf基因在胰腺导管上皮中的表达情况,对新鲜小鼠胰腺组织切片进行显微切割,分离纯化胰腺组织中的导管和胰岛,以及外分泌腺组织细胞作为对照,利用荧光实时定量PCR的方法完成对目的基因的相对定量.结果显示,mafamRNA,mafbmRNA水平在胰岛及导管中非常接近,无统计学差异.而c-maf在导管的表达高于胰岛(P<0.05),外分泌腺则无上述基因的表达.胰腺导管中mafa,mafb,cmaf均有表达,肯定了导管上皮细胞向内分泌细胞分化的潜能,而c-maf在导管中的表达高于胰岛,提示导管上皮c-maf的下调可能有助于导管上皮细胞向内分泌细胞的分化成熟.As a candidate for progenitor of endocrine cells, pancreatic duct epithelium displays more and more importance of differentiation potential to insulin-producing cells. Some cell markers such as pdx-1, nkx6.1 expressed in pancreatic islet development have also been found in duct. The maf family, which is a subgroup of the basic leucine zipper（bZip） transcription factors with homology to the v-Maf oncoprotein, the founding member of the Maf family, which was originally identified in the genome of the AS42 chicken musculoaponeurotic sarcoma retrovirus in 1989, has been involved in pancreatic research recently. Members of Maf family, mafa, mafb, c-maf, which have been reported to be regulators of tissue-specific gene expression and cell-differentiation in a wide variety of tissues, are all expressed in islet and regulate insulin expression as well as islet development. Whether these genes are expressed in duct remains unclear and demands further investigation. Islet, duct and exocrine tissues were acquired by laser-capture microdissection from mouse pancreatic section, followed by real-time PCR to quantitate the target genes expression. Mafa, mafb, c-maf were all detective in duct and islet but none was in exocrine tissue. In addition, c-maf mRNA level in duct was higher than islet（0.300±0.018 vs 0.796±0.173, P 〈 0.05）, however, there was no difference in mafa and mafb mRNA level between islet and duct. The expression of mafa, mafb, c-maf in pancreatic duct epithelium, gives more support to not only the roles of the three genes in endocrine development, but also the potential of converting duct epithelium to endocrine cells. Moreover, the higher expression of c-maf in duct than islet suggests that down-regulation of c-maf might help to differentiation and mature of endocrine cells.

关 键 词：激光显微切割 胰腺导管上皮细胞 maf家族基因 实时定量PCR 

分 类 号：R587.1[医药卫生—内分泌] R322.57[医药卫生—内科学]