NOR1 基因转染对肝癌细胞 HepG2 基因表达谱的影响  

Changes in Global Gene Expression Induced by NOR1 Over-expression in HepG2 Cells

作　　者：李登清[1] 唐华[1] 桂嵘[2] 聂新民[2] 

机构地区：[1]中南大学湘雅医学院医学检验系,长沙410013 [2]中南大学湘雅三医院检验科,长沙410013

出　　处：《生物化学与生物物理进展》2008年第4期457-464,共8页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(30300383);中国博士后科学基金(2004035206);湖南省自然科学基金(07JJ3036)资助项目~~

摘　　要：NOR1基因是一在正常组织中广泛表达且在肿瘤组织中表达下调的新基因.为进一步研究NOR1基因的功能和寻找其下游基因,利用脂质体技术将NOR1基因转染进HepG2细胞,采用cDNA微阵列技术分析其基因表达谱的改变.试验表明NOR1基因的转染能使Grb2,HBP17,TNFRSF11B等59个基因上调,同时也下调Bik,MAP2K6,ZFP95等103个基因.随后用实时荧光定量PCR对cDNA微阵列结果中上述3个上调表达基因进行验证,结果表明,基因表达差异具有统计学意义(P<0.05),荧光定量PCR结果与微阵列结果相符.这些结果提示,NOR1基因对肝癌HepG2细胞的生物学行为的影响可能与它对细胞信号转导,细胞周期调控,转录、翻译调控相关基因的表达影响有关.Previous work from this laboratory has cloned a novel gene NOR1 and showed its extensive expression in normal tissues and down-regulation in carcinomas. To further investigate its downstream target genes and better understand its function, NOR1 was over-expressed in HepG2 hepatoma cells and global changes in gene expressions from a stable line were identified by cDNA microarrays. The results discovered 59 genes up-regulated in these cells compared with the original cells, including Grb2, HBP17, TNFRSF11B genes that have been implicated in tumorigenesis and cancer development. In addition, 103 down-regulated genes were also identified, including genes encoding Bik, MAP2K6 and ZFP95 proteins. The expression patterns of certain genes identified by microarrays were validated by quantitative real-time PCR and the results showed that expression difference were statistically significant （P 〈 0.05）. These data suggest that NOR1 may influence the biology and cancerous behaviors of HepG2 cells by regulating expression of a set of genes involved in signal transduction, cell cycle regulation, transcription and translation controls.

关 键 词：NOR1基因 肝癌 CDNA微阵列 实时荧光定量PCR 

分 类 号：R397.63[医药卫生—基础医学]