野生花卉老鹳草的引种和植物组织培养研究  

The wild flower Geranium thunbergii Siebet Zuce leads a kind Study with plant tissue culture

作　　者：贠剑[1] 

机构地区：[1]山西农业大学林学院,山西太谷030801

出　　处：《山西农业科学》2008年第4期44-46,共3页Journal of Shanxi Agricultural Sciences

摘　　要：实验用老鹳草无菌苗做原料,利用老鹳草的茎为外植体,进行离体培养,选出合适的培养基,以培育出品质好的完整植株,为以后的引种驯化提供试验材料。结果表明,培养基灭菌时间为3min,琼脂浓度为7g.L-1时,种子的出芽率为最高,可达100%;MS+6-BA 0.3mg.L-1+NAA 0.1mg/l.L-1的培养基为茎段离体培养的最佳培养基。This experiment has no germ seedling to do raw material with Geranium thunbergii Siebet Zuce and make use of the caulis of Geranium thunbergii Siebet Zucein order to outside plant a body, carry on leaving body development, select a suitable culture media to grow product the quality good complete plant, is hereafter of lead a kind to tame to tufa to provide experiment material. Express that culture media＇s putting out germ is 3 minutes for time as a result, the Qiong fat density is a 7 gs ·point L^-1, of the seed bud rate is tallest, can reach to 10096; MS＋6-the BA 0.3 tugs ·L^-1＋ NAAs 0.1 mgs/l · the culture media of L^-1 for caulis segment the best culture media which leave body development.

关 键 词：老鹳草 无菌苗 外植体 培养基 离体培养 

分 类 号：S322.3[农业科学—作物遗传育种]