不同草种对土著AM真菌的生长和群落结构的影响  

Influence of different grass species on growth and community structure of native AM fungi

作　　者：姚青[1] 王连润[1] 陈美标[2] 胡又厘[1] 朱红惠[2] 王燕[3] 

机构地区：[1]华南农业大学园艺学院,广东广州510642 [2]广东省微生物研究所广东省微生物菌种保藏中心,广东广州510070 [3]广东省科技图书馆,广东广州510070

出　　处：《草业学报》2008年第2期33-38,共6页Acta Prataculturae Sinica

基　　金：国家自然科学基金(30200006);广东省自然科学团队基金(E05202480)资助

摘　　要：以果园生草栽培为背景,果园土壤中土著AM真菌为研究对象,考察了柱花草、百喜草和藿香蓟对与之根系共生的AM真菌的生长与群落结构的影响。结果表明,柱花草根系的侵染率(62.2%)显著高于百喜草(36.5%)和藿香蓟(37.3%),丛枝率有相似的趋势;3种草地上部的生物量和含磷量没有差异,但是柱花草根系含磷量显著高于百喜草和藿香蓟;柱花草根际土壤中AM真菌的菌种丰度和菌丝密度均小于百喜草和藿香蓟,但是孢子密度大于藿香蓟而小于百喜草;藿香蓟根际土著AM真菌群落的多样性指数略高于柱花草和百喜草;PCR-DGGE分析结果与之吻合。试验表明,在果园土壤中,不同草种根际的土著AM真菌群落结构并不相同,在选择草种进行生草栽培的过程中应该考虑这些不同。With sod culture in orchards as background and the native arbuscular mycorrhizal （AM） fungi in orchard soil as target, the influences of Stylosanthes gracilis, Ageratum conyzoides and Paspalum notatum on the growth and community structure of AM fungi were investigated. Results showed that the root colonization rate of S. gracilis （62.2%） was higher than that of A. conyzoides （36.5%） and P. notatum （37.3%）. The arbuscule percentage showed a similar pattern. Although the biomass and P content in the shoots of the three grass species were comparable, P content in S. gracilis roots was higher than that in A. conyzoides and P. notatum roots. The species richness and hyphal density in the rhizosphere soil of S. gracilis plants were less than those of A. conyzoides and P. notatum plants, while the spore density was highest for A. conyzoides and lowest for P. notatum. Diversity index of the native AM fungi in the rhizosphere soil of P. notatum plants was slightly higher than those of S. gracilis and A. conyzoides plants. PCR-DGGE analysis was consistent with the morphological results. Our results indicate that the community structure of native AM fungi in orchard soil differed from each other when colonized with different grass species, which should be taken into account when selecting appropriate grass species for sod culture.

关 键 词：柱花草 百喜草 藿香蓟 丛枝菌根真菌 群落结构 

分 类 号：S181[农业科学—农业基础科学]