异钩藤碱体外对家兔血小板聚集和胞浆游离钙离子浓度的影响  

Effect of isorhynchophylline on platelet aggregation and cytoplasmic free calcium level in rabbit platelets in vitro

作　　者：谢笑龙[1] 吴敏[1] 吴芹[1] 黄燮南[1] 龚其海[1] 石京山[1] 

机构地区：[1]遵义医学院药理学教研室,贵州遵义563000

出　　处：《中国药理学与毒理学杂志》2008年第2期116-119,共4页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的研究异钩藤碱对血小板内游离钙离子浓度（[Ca^2＋]i）的影响，以探讨其抗血小板聚集作用的可能机制。方法比浊法测定家兔血小板聚集功能；双波长Fura-2荧光法测定血小板胞浆[Ca^2＋]i。结果异钩藤碱0．33～1．30mmol·L-1体外给药对ADP和凝血酶引起的血小板聚集有浓度依赖性的抑制作用。存在细胞外钙时，异钩藤碱对基础状态血小板的[Ca^2＋]i和ADP及凝血酶诱导的[Ca^2＋]i水平有浓度依赖性的降低作用，而无细胞外钙存在时，则均无明显影响，表明其可抑制血小板的外钙内流，对内钙释放无明显抑制作用。结论异钩藤碱可抑制血小板聚集，其作用机制可能与其抑制血小板胞浆[Ca^2＋]i升高有关。AIM Observing the effect of isorhynchophylline on cytoplasmic free calcium level （ [Ca^2＋]i ） of platelets, to investigate the possible mechanism of its anti-platelet aggregation. METHODS Rabbit platelet aggregation and platelet [Ca^2＋]i were determined by Born method and double beam fluorescence spectrophotometric method, respectively. RESULTS Isorhynchophylline 0. 33 - 1. 30 mmol. L-1 administered in vitro visibly inhibited the rabbit＇s platelet aggregation induced by ADP （15μmol.L^-1） and thrombin （3 kU.L-1） in a concentration-dependent manner. In the presence of extracellular [Ca^2＋]i, isorhynchophylline inhibited the resting [Ca^2＋]i and the increased [Ca^2＋]i induced by ADP （ 15 μmol· L^-1 ） and thrombin （3 kU.L^-1） in a concentration-dependent manner, but had no effect on [Ca^2＋]i in the absence of extracellular Ca^2＋, which indicated that isorhynchophylline inhibited extracellular Ca^2＋ influx in platelets, but had no inhibitory effect on intracellular Ca^2＋ mobilization. CONCLUSION Isorhynchophylline markedly inhibits the rabbit platelet aggregation, and the mechanism may be related to its depression on the rise of platelet [Ca^2＋]i.

关 键 词：异钩藤碱 血小板聚集 钙 细胞内 凝血酶 

分 类 号：R285[医药卫生—中药学]