二花脸与杜洛克猪繁殖相关基因表达差异  

Expression Differences in Genes Related to Reproduction Between Erhualian and Duroc Pigs

作　　者：杜红丽[1] 陈静[2] 张玉山[2] 崔建勋[3] 张细权[2] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510640 [2]华南农业大学动物科学学院,广东广州510642 [3]广东省农业科学院科技情报研究所,广东广州510640

出　　处：《华南农业大学学报》2008年第2期99-103,共5页Journal of South China Agricultural University

基　　金：国家973项目(G2000016102)

摘　　要：以二花脸和杜洛克猪种为试验材料,采用real-time定量PCR技术,对猪ESR、RBP4、PRLR、INHA、SPP1、DPP4、P450arom、LHR、FSHR、FSHβ、GnRHR、EGF、PAPP-A、IGF1、NRP2、GDF9和SCG2等17个与繁殖性能相关基因在这2个猪种垂体、卵巢、子宫、输卵管4个组织中的表达差异进行研究.结果显示在17个基因中有15个基因(ESR、RBP4、PRLR、INHA、DPP4、P450arom、FSHR、FSHβ、GnRHR、EGF、PAPP-A、IGF1、NRP2、GDF9和SCG2)在二花脸子宫中表达量均高于杜洛克子宫的表达量,尤以RBP4(相对定量结果为123.35)和ESR(相对定量结果为10.67)表现最为明显;而垂体除了ESR和GDF9在二花脸中表达量(7.15和5.47)明显比杜洛克高之外,其余基因在二花脸中的表达量与杜洛克的基本差不多,甚至表达量还少些(如P450arom、LHR、IGF1、SPP1、RBP4);17个基因在两猪种卵巢及输卵管的表达差异不明显.结果提示二花脸猪高产仔性能是受多个基因综合作用的结果,而且可能主要受子宫内基因表达水平的影响.Real-t/me quantitative PCR was applied to analyze expression differences in genes related to reproduction between Erhualian and Duroc pigs. Seventeen genes （ESR, RBP4, PRLR, INHA, SPP1, DPP4, P450arorn, LHR, FSHR, FSHβ, GnRHR, EGF, PAPP-A, IGF1, NRP2, GDF9 and SCG2 ） were analyzed in the present study in four tissues （pituitary, ovary, uterus and oviduct） of the two pig breeds. The results showed that the expression level of each of the 15 genes （ESR, RBP4, PRLR, INHA, DPP4, P450arom, FSHR, FSHβ, GnRHR, EGF, PAPP-A, IGF1, NRP2, GDF9 and SCG2） was higher in Erhualian uterus than in Duroc uterus. The relative amounts of RBP4 and ESR were 123.35 and 10. 67, respectively in Erhualian uterus, which were much higher than those （1.0） of the two genes in Duroc uterus. In pituitary, the relative amounts of ESR and GDF9 were 7. 15 and 5. 47, respectively in Erhualian, which were higher than those （1.0） of the two genes in Duroc; the relative amounts of P450arom, LHR, IGF1, SPP1 and RBP4 were lower in Erhualian than those in Duroc ; and the relative amounts of the other genes were almost the same in the two breed pigs. There was no significant difference in the relative amounts of the 17 genes in the ovary and oviduct tissues between Erhualian and Duroc. The results in the present study implied that the high fertility of Erhualian was probably due to complicated effect of many genes, and most parts were possibly affected by the expression of genes in uterus tissue.

关 键 词：二花脸 杜洛克 繁殖 基因表达差异 

分 类 号：S813.1[农业科学—畜牧学]