生长因子对培养人眼视网膜色素上皮细胞增殖的调控作用  

The modulation of cell proliferation induced by growth factors in human retinal pigment epithelial cells

作　　者：曹景泰[1,2] 张惠蓉[1,2] 刘宁朴[1,2] 武丽 白若昀[1,2] 

机构地区：[1]北京医科大学第三医院眼科 [2]石家庄白求恩国际和平医院电镜室

出　　处：《中华眼科杂志》1997年第5期360-362,共3页Chinese Journal of Ophthalmology

基　　金：国家教委博士点科研基金

摘　　要：目的探讨生长因子对人眼视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉｕｍ，ＲＰＥ）细胞增殖的调控作用。方法建立人眼ＲＰＥ细胞培养体系，利用３Ｈ－ＴｄＲ掺入法和细胞计数观察表皮生长因子（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＥＧＦ）、碱性成纤维细胞生长因子（ｂａｓｉｃ－ｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂ－ＦＧＦ）、胰岛素样生长因子（ｉｎｓｕｌｉｎ－ｌｉｋｅｇｒｏｗｔｈｆａｃｔｏｒ，ＩＧＦ－Ｉ）对培养ＲＰＥ细胞的作用。结果（１）ＥＧＦ，ｂ－ＦＧＦ，ＩＧＦ－Ⅰ与对照组比较，可明显增强人眼ＲＰＥ细胞ＤＮＡ合成６．６～１２．２倍，增强能力为ＥＧＦ＞ｂ－ＦＧＦ＞ＩＧＦ－Ⅰ。（２）当生长因子联合作用时，可数倍明显增强３Ｈ－ＴｄＲ掺入，能较单一因子更有效地刺激人眼ＲＰＥ增殖。结论结果提示，生长因子的协同作用可能是增殖性视网膜病变发生的重要机理之一。Objective To explore the modulation of cell proliferation induced by growth factors in cultured human retinal pigment epithelial (RPE) cells. Methods A well cultured system of human fetal RPE cells was established to determine the effects of several growth factors, such as epidermal growth factor (EGF), basic fibroblast growth factor (b FGF), insulin like growth factor (IGF I), on the cultured human RPE cells by using 3H thymidine incorporation and cell count. Results (1) EGF, b FGF, IGF I significantly increased the DNA 6.6～12.2 times in human RPE cells in comparison with the control, the rank order of potency of the factors being EGF>b FGF>IGF I. (2) When the factors were used in combination, the above factors caused a significant enhancement of 3H Tdr incorporation by times. Conclusion It is suggested that the synergetic effects of growth factors and their complex interactions play a role in the development of proliferative retinopathy.

关 键 词：视网膜色素上皮 生长因子 细胞增殖 视网膜病变 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R774.12[医药卫生—基础医学]