重组人白介素-6(rhIL-6)对γ线照射小鼠外周血细胞恢复的影响  

EFFECT OF DIFFERENT DOSES OF rhIL 6 ON PERIPHERAL BLOOD RECOVERY IN IRRADIATED MICE

作　　者：邱丽玲[1] 柳晓兰[1] 孙连生[1] 张卿西[1] 赵振虎[1] 王嘉玺 凌世淦 毛秉智[1] 

机构地区：[1]北京放射医学研究所

出　　处：《解放军医学杂志》1997年第4期250-252,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：在８０～９０ｄ日龄Ｃ５７ＢＬ／６Ｊ小鼠上观察了１０～１０００μｇ／ｋｇｄ－１４种剂量ｒｈＩＬ－６对６．５Ｇｙγ线（１３２～１２７．７Ｒ／ｍｉｎ）照射后外周血细胞恢复的影响；观察时间持续至照射后３０天。结果表明：白细胞、红细胞和血小板计数的动态变化时间进程给药组和对照组相似，但总的趋势表明给药组各种血细胞计数的最低值高于对照组、各时间点的恢复情况均不同程度地优于对照组；除１０μｇ组外均有一部份时间点统计学差别显著，而５００和１０００μｇ剂量组效果尤为显著。血红蛋白含量和ＲＢＣ比积的变化与ＲＢＣ数一致。相关系数分析结果表明在一部分时间点上，外周血细胞数有明显的药量依赖性。照后２２天ＷＢＣ数、１４和１８天ＲＢＣ数以及１１和１８天ＰＬＴ数的剂量效应回归方程均具有统计学意义。本文结果表明：皮下注射ｒｈＩＬ－６剂量达到２００μｇ／ｋｇｄ－１以上连续４ｄ可减轻６．５Ｇｙ照射小鼠外周血细胞数下降程度并促进其恢复。其机制可能是通过ＩＬ－６对造血干、祖细胞增殖与分化的改善而起作用。The effects of recombinant human interleukin 6(rhIL 6) on the recovery of peripheral blood picture in 6 5Gy γ ray irradiated (132 127.7R/min)C 57 BL/6J mice at the age of 80 90 days were studied. Group 1 served as the control, and Groups 2 5 were injected subcutaneously with rhIL 6 b.i.d at doses of 10, 200, 500 and 1000μg/kg·d -1 , respectively, started at 30 min after irradiation for 4 consecutive days. Peripheral WBC,RBC and PLT counts, HGV and HCT were determined before radiation and 5, 11, 14, 18, 22 and 30 days after radiation.It was shown that although the time of nadir and the onset of recovery were similar in all the 5 groups, the nadir values were higher and the recovery was better, both dose dependent, in the IL 6 treated groups than in the control. Significant dose response regression equations were obtained for WBC count on day 22, for RBC count on day 14 and 18; for PLT count on day 11 and 18. The results show that rhIL 6 injected subcutaneously at doses≥200 μg/kg·d -1 for 4 consecutive days could reduce radiation induced peripheral blood cell injury and promote its recovery in 6 5 Gy γ irradiated mice. Possibly, this is due to the beneficial effect of rhIL 6 on the proliferation and differentiation of hemopoietic stem cells and progenitor cells.

关 键 词：RHIL-6 Γ线照射 外周血细胞计数 恢复 

分 类 号：R818.05[医药卫生—放射医学]