家庭内乙型肝炎病毒变异株感染的分子病毒学调查  

HEPATITIS B VIRUS MUTATIONS IN FAMILY CLUSTERED CHINESE PATIENTS

作　　者：侯金林[1] 王战会[1] 林华[1] 骆抗先[1] 梁炽森[1] 钟梅[1] 章廉[1] 杨守昌[1] 陈家芳 

机构地区：[1]第一军医大学南方医院 [2]福州空军医院

出　　处：《解放军医学杂志》1997年第4期263-265,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家和广东自然科学基金

摘　　要：为分析乙型肝炎病毒（ＨＢＶ）家庭内聚集感染病例的ＨＢＶ变异特点，我们对１０个家庭２５例ＨＢＶ感染者进行了分子病毒学研究，均用单链构型多态性分析（ＳＳＣＰ）ＨＢＶ外膜基因“ａ”决定簇片段的变异，ＰＣＲ－限制性片断长度多态性分析，分析ＨＢＶ前Ｃ区终码变异。结果与用聚合酶链反应（ＰＣＲ）产物直接序列分析测定的４个家庭有、无ＨＢＶ变异株感染的外膜基因序列和前Ｃ基因序列比较，从分子水平鉴定ＨＢＶ家庭内传播，同时建立了ＳＳＣＰ检测ＨＢＶ免疫逃避变异株的方法。In order to determine the molecular characterization of hepatitis B virus mutation in family clustered Chinese patients, 25 cases of such individuals from 10 HBV infection clustered Chinese families were investigated. The presence of 'a' determinant variant was detected by a single strain conformation polymorphism assay based on polymarase reaction amplified DNA. The precore stop codon mutant was detected with a mismatched PCR in combination with a restriction fragment length polymorphism assay. The result was verified by direct sequencing in 4 HBV clustered families. HBV transmission linkage was established in HBV infection clustered families on a molecular level. We also firstly described a new method for detecting HBV immune escape mutant in Chinese vaccinated individuals.

关 键 词：乙型肝炎病毒 乙型肝炎 表面抗原 分子生物学 

分 类 号：R512.62[医药卫生—内科学]