大豆“南农94-16”突变体库的构建及部分性状分析  

CONSTRUCTION OF MUTANT LIBRARY FOR SOYBEAN "Nannong 94-16" AND ANALYSIS OF SOME CHARACTERS

作　　者：韩锁义[1] 杨玛丽[1] 陈远东[1] 于静静[1] 赵团结[1] 盖钧镒[1] 喻德跃[1] 

机构地区：[1]南京农业大学大豆研究所/国家大豆改良中心,作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《核农学报》2008年第2期131-135,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家“973”项目(2004CB117206);“863”项目(2006AA10Z1C1);国际原子能机构项目(CRP12988)

摘　　要：本研究对"南农94-16"大豆种子用NaN3-60Coγ射线复合诱变以及EMS诱变来构建大豆突变体库。两种方式诱变大豆分别获得54份和66份叶、茎、花、种子、子叶等性状变异的突变体。用644对SSR标记分别对其中的14株耐涝突变体和1株叶色浅绿突变体进行了鉴定。结果表明,14株耐涝突变体,有2株与对照有超过100个标记的差异,其余植株分别有1到7个标记的差异;叶色浅绿突变体与对照有39个标记的差异,遗传分析表明该性状由细胞核单基因隐性遗传控制。这些突变体可以作为新的种质资源,构建的突变体库也有助于大豆功能基因组研究的开展。The soybean mutant library was created by treating the seeds of soybean ＂Nannong 94-16＂ with NaN3-^60 Co γ-rays irradiation combination treatment and EMS, inspectively. In M3 generations, 54 mutants of leaf, stem, flower, seed and cotyledon mutants were obtained by treatment of NaN3-^60 Co γ-rays irradiation, and 66 mutants by EMS, inspectively. Water- logging tolerance mutants of 14 and the light green leaf color mutant were identified by 644 SSR primer pairs. The results showed that, among 14 water-logging tolerance mutants, more than 100 primer pairs displayed polymorphism in 2 mutants and one to seven SSR primer pairs displayed polymorphism in other plants; for the light green leaf color mutant, 39 primer pairs displayed polymorphism. Genetic analysis to the light green leaf color mutant showed that this character was controlled by one pair of recessive nuclear genes. The mutant library created for ＂Nannong 94-16＂ will be helpful for the research in soybean functional genomics.

关 键 词：大豆 NaN3-^60Co γ射线复合诱变 EMS诱变 突变体 

分 类 号：S565.1[农业科学—作物学]