枣幼胚子叶再生植株的研究  

PLANT REGENERATION FROM COTYLEDON OF IMMATURE EMBRYO OF JUJUBE

作　　者：王国平[1] 李晓梅 马会勤[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100094 [2]山西农业科学院果树研究所,山西太谷030815

出　　处：《核农学报》2008年第2期152-155,147,共5页Journal of Nuclear Agricultural Sciences

摘　　要：本试验研究了影响金丝小枣幼胚子叶再生植株的相关因素,获得了金丝小枣幼胚子叶的再生植株。正交试验结果表明:金丝小枣幼胚子叶在附加TDZ0.6mg/L、IAA0.5mg/L、NAA0.3mg/L的1/2MS培养基中,暗培养20d,愈伤组织诱导率和再生频率均最高,分别达到100%和41.86%。再生获得的不定芽在MS培养基上,添加6-BA1.0mg/L和IBA0.1mg/L,增殖效果最好;在1/2MS+IBA1.0mg/L培养基上,生根率达到100%。The effects of TDZ, IAA, NAA and medium on bud initiation of cotyledon of immature embryo from jujube cultivar Jinsixiaozao were evaluated by L9 （3^4） orthogonal design. The result showed that TDZ was the most important factor for bud initiation followed by medium, IAA, and NAA. The optimum combination medium was on 1/2MS medium containing TDZ （0.6mg/L）,IAA（0.5mg/L）and NAA0.3（mg/L） by,variance analysis, on which cultured in dark 20d, callus inducing rate was 100 % and adventitious bud inducing rate was 41.86 %. The optimum media for adventitious bud propagation, and for root inducing are on MS medium adding 6-BA 1.0mg/L, IBA 0. lmg/L and on 1/2MS medium supplemented with IBA 1.0mg/L respectively.

关 键 词：枣 幼胚子叶 不定芽再生 

分 类 号：S665.1[农业科学—果树学]