高效液相色谱法测定异烟肼注射液的含量  

Content Determination of Isoniazid Injection by HPLC

作　　者：黎昌权 

机构地区：[1]重庆市万州药品检验所,重庆万州404000

出　　处：《中国药业》2008年第8期32-33,共2页China Pharmaceuticals

摘　　要：目的探讨用高效液相色谱(HPLC)法测定异烟肼注射液的含量。方法采用DiamonsilC18色谱柱(250mm×4.6m m,5μm),流动相为甲醇-水(20∶80),检测波长为263nm,流速为1.0mL/min。结果进样量与峰面积的线性范围为0.202～1.01μg(r=0.9998),平均回收率为99.53%,RSD为0.36%(n=6)。结论HPLC法简便、准确、灵敏度高,重现性好,可用于异烟肼注射液的含量测定。Objective To establish a method for the determination of Isoniazid Injection by HPLC. Methods Diamonsil C18 5μ column（250mm × 4.6 μm）was used. The mobile phase was methanol-water（20 ： 80）.The detection wavelength was set at 263 nm, the flow rate was 1.0 mL/min. Results The linear range of hydrochloride was 0. 202 - 1.01 μg（ r =0. 999 8）, the average recovery rate was 99. 53%, RSD was 0. 36% （n=6）. Conclusion The method is simple, and accurate with, higher sensitivity and good reproducibility. This method can be used for the content determination of Isoniazid Injection.

关 键 词：高效液相色谱法 异烟肼注射液 含量测定 

分 类 号：R927.2[医药卫生—药学] R978.3