龙眼胚性培养物APX同工酶的分析方法建立及其在龙眼体胚发生过程中的变化  

A method for analyzing APX isozymes from embryogenic cultures by polyacrylamide gel electrophoresis and the changes of APX isozymes at the stages of somatic embryogenesis in longan

作　　者：邵巍[1] 赖钟雄[1] 赖呈纯[1] 孙云[1] 陈义挺[1] 蔡英卿[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2008年第2期140-144,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省自然科学基金资助项目(C0410012);国家自然科学基金资助项目(30471204)

摘　　要：以龙眼胚性培养物为材料,进行抗坏血酸过氧化物酶(APX)同工酶的聚丙烯酰胺凝胶电泳分析方法研究,建立一套适合分离龙眼胚性培养物中不同类型APX同工酶的垂直板聚丙烯酰胺凝胶电泳方法;进一步研究了APX同工酶在龙眼体胚发生过程中的变化,发现在胚性愈伤组织、球形胚、子叶形胚3个阶段APX同工酶存在差异.A simple and efficient method was developed for analyzing APX isozymes from synchronized embryogenic cultures of longan by polyacrylamide gel electrophoresis. Then, using the method, the changes of APX isozymes at different stages of longan somatic embryogenesis were analyzed. The results showed that there were significant differences of the APX isozymes between the embryogenic callus stage, the globular embryo stage and the cotyledon embryo stage of longan somatic embryogenesis.

关 键 词：龙眼 体胚发生 抗坏血酸过氧化物酶 同工酶 电泳 

分 类 号：S667.2[农业科学—果树学]