兔胸腺HMGN2的分离纯化鉴定  

Separation and identification of rabbit thymus HMGN2

作　　者：石艳娥[1] 李恒[1] 彭媛媛[1] 邓璐霞[1] 周新娥[1] 赵媛[1] 黄宁[1] 

机构地区：[1]四川大学华西医学中心基础医学与法医学院感染免疫研究室,成都610041

出　　处：《四川生理科学杂志》2008年第1期1-2,共2页Sichuan Journal of Physiological Sciences

基　　金：国家自然科学基金资助项目(30671963;30470763);纽约中华医学会基金(98-681)

摘　　要：目的:分离纯化鉴定兔高迁移率非组蛋白N2(High mobility group chromosomal protein N2,HMGN2)。方法:利用液氮研磨和5%高氯酸从新鲜兔胸腺组织获得酸溶性萃取物,经反相高效液相色谱(RP-HPLC)分离得到HMGN2,经过SDS-聚丙烯酰胺凝胶电泳和Western-blotting对HMGN2进行分子量测定和特异性鉴定。并利用琼脂糖弥散抑菌实验对其杀菌活性进行鉴定。结果:利用上述方法分离纯化出高纯度的兔HMGN2。对大肠埃希菌临床分离株54080、大肠埃希菌ATCC25922有明显杀菌活性。结论:建立简单分离高纯度的HMGN2的方法。Objective.. To separation and identification of rabbit thymus HMGN2. Methods： Fresh rabbit thymus are grinded by liquid nitrogen, then extracted by 5% perchloric acid. The extractant separated by RP-HPLC, and sodium dodecyl sulfate polyacryl- amide gel electrophoresls identification for HMGN2 and determination molecular weight. Specificity identification by Western-blot- ting. Results： We have got high purified HMGN2. ConciSion： We find a simple and direct way of separating and purifying nature HMGN2, which will be useful for the research of HMGN2.

关 键 词：兔胸腺 HMGN2 分离 

分 类 号：R392[医药卫生—免疫学]