“劳倦过度、房室不节”肾阳虚模型小鼠睾丸乳酸脱氢酶-X活性的测定  

Determination of LDH-X activity in testes of mice with deficiency of Kidney-yang induced by overstrain and intemperate Sexual Intercourse

作　　者：李震[1] 刘黎青[1] 颜亭祥[2] 李淑燕[1] 艾邸[1] 宋辉[2] 闫川慧[2] 王媛[2] 慕小婧[2] 刘鹏[2] 郭根艳[2] 

机构地区：[1]山东中医药大学基础医学院,济南250014 [2]山东中医药大学,济南250014

出　　处：《天津中医药》2008年第2期136-138,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金重大研究计划资助项目(项目批准号:90209014)

摘　　要：[目的]观察"劳倦过度、房室不节"肾阳虚小鼠睾丸乳酸脱氢酶(LDH)总活性及精子特异酶LDH-X活性的改变。[方法]睾丸匀浆用2,4-二硝基苯肼显色法测定丙酮酸的生成量[1],计算每克睾丸湿组织LDH总活性和LDH-X同工酶活性。[结果]"劳倦过度、房室不节"肾阳虚造型组LDH总活性显著低于正常对照组(P<0.05,P<0.01),金匮肾气丸治疗组较"劳倦过度、房室不节"肾阳虚造型组则显著升高(P<0.05,P<0.01);LDH-X活性造型组第2周较对照组有下降的趋势,但无统计学意义,第4、第6周下降显著(P<0.01);治疗组较造型组第2周有升高趋势,但无统计学意义,第4、第6周则显著升高(P<0.01)。[结论]从精子特异酶LDH-X活性改变,证明了"肾主生殖"有现代科学依据,该模型系肾阳虚模型。[Objective] The changes of LDH and LDH-X activity in testes of mice with deficiency of kidney-yang induced by overstrain and intemperate sexual intercourse were examined. [Methods] The contents of pyruvate in testis homogenates was measured and the total LDH activity and LDH-X isoenzyme activity per gram of wet tissue were determined by 2,4-dinitrophenylhydrazine color test in different groups. [Results] The total LDH activity in model group of deficiency of kidney-yang was significantly lower than that in normal control group （P〈0.05, P〈0.01 respectively）while it was higher in therapy group with Jingui Shenqi pill P〈0.05, P〈0.01 ）compared with that in model group. LDH-X activity in the second week in model group was decreased compared with that in control group, but there is no statistical difference. Until the fourth and sixth week the decrease became significant （P〈0.05）;Compared with LDH-X activity in model group, it was raised in the second week in therapy group, but statistics showed no significance. But it was increased significantly in the fourth and sixth week（P〈0.01 ）. [Conclusions] The ground of modern science of ＂the kidney dominating reproduction＂ is proved by the changes of LDH-X （specific enzyme of sperm） activity and this model is the one with deficiency of kidney-yang.

关 键 词：肾阳虚 小鼠 乳酸脱氨酶 乳酸脱氢酶-X 

分 类 号：R587.1[医药卫生—内分泌]