滋肾调冲法对诱发排卵大鼠卵巢血管内皮生长因子表达的影响  

Effects of Zishen Tiaochong method on ovaries VEGF expression in rats with induced ovulation

作　　者：李瑞环[1] 王玲[1] 孙艳明[1] 徐瑾[2] 陈静[1] 张远[1] 

机构地区：[1]天津中医药大学,研究生天津300193 [2]天津医院,天津300211

出　　处：《天津中医药》2008年第2期144-146,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：天津市卫生局科研资助项目(03033)

摘　　要：[目的]观察滋肾调冲法对诱发排卵大鼠卵巢血管内皮生长因子(VEGF)表达的影响。[方法]将80只大鼠随机分成功血宁Ⅱ号高、低剂量组,阳性对照组和模型组4组,免疫组化法检测各组卵巢卵泡发育期和排卵前期VEGF表达水平。[结果]功血宁Ⅱ号组阳性面积、平均光密度、阳性面积率、积分光密度在卵泡发育期和排卵前期均高于模型组;排卵前期各组VEGF表达均高于卵泡发育期。[结论]滋肾调冲法促卵泡发育及排卵的作用机制可能与提高卵巢VEGF的表达、改善卵泡的血供有关。[Objective] To observe the effects of Zishen Tiaochong method on ovaries VEGF expression of rats with induced ovulation. [Methods] Eighty rats were randomly divided into four groups： the high-dose and low-dose of Gongxuening Ⅱ group, positive control group and model group. The VEGF expression at ovary follicular development stage and preovulatory stage of four groups was detected with immunohistochemistry. [Results] During follicular development stage and preovulatory stage, the area, MOD, area%, IOD of Gongxuening Ⅱ group were obviously higher than that of model group. The VEGF expression of preovulatory stage was all higher than that of follicular development stage in four groups. [Conclusions] The molecular mechanism of Zishen Tiaochong Method is possibly concerned with the promotion on ovarial VEGF expression and improvement of follicular blood supply.

关 键 词：滋肾调冲法 血管内皮生长因子 免疫组化法 实验研究 

分 类 号：R285.5[医药卫生—中药学]