4种终末宿主土耳其斯坦东毕吸虫cox1和nad1基因的序列分析  

Sequence analysis of cox1 and nad1 genes of Orientobilharzia turkestanicum from 4 definitive hosts

作　　者：李利[1] 邢继兰[2] 王春仁[1] 翟延庆[1] 何国声[3] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]上海市农业科学院畜牧兽医研究所,上海201106 [3]中国农业科学院上海兽医研究所,上海200232

出　　处：《中国兽医科学》2008年第4期303-307,共5页Chinese Veterinary Science

基　　金：黑龙江省教育厅科学技术研究项目(11511253)

摘　　要：收集黑龙江省大庆地区牛源、绵羊源、绒山羊源和山羊源土耳其斯坦东毕吸虫，以SDS-蛋白酶K法抽提其基因组DNA，用特异性引物通过PCR扩增土耳其斯坦东毕吸虫成虫细胞色素C氧化酶亚基1基因（coxl）和烟酰胺腺嘌呤二核苷酸脱氢酶亚基1基因（nad1）并进行测序，应用Chromas和DNAStar软件分析扩增产物的变异情况。结果表明，大庆地区4种终末宿主土耳其斯坦东毕吸虫cox1基因均为1125bp，nad1基因均为518bp；且其线粒体基因存在差异，coxl的同源性为99．0％～99．7％，nad1的同源性为98．3％～99．4％。Orientobilharzia turkestanicurn were isolated from cattle, sheep, cashmere goat and native goat in Daqing Prefecture of Heilongjiang Province, China,and their genomic DNAs were extracted by SDSprotease K method. The cox1 and nad1 genes were amplified by PCR,sequenced,and analyzed by Chromas and DNAStar softwares. The results showed that the lengths of cox1 and nad1 sequences from the 4 definitive hosts of O. turkestanicurn were 1 125 bp and 518 bp,respectively. The homology of cox1 gene was from 99.0% to 99.7% and homology of had1 gene was from 98.3~ to 99.4~ among the 4 definitive hosts.

关 键 词：土耳其斯坦东毕吸虫 细胞色素C氧化酶亚基1基因 烟酰胺腺嘌呤二核苷酸脱氢酶亚基1基因 序列分析 

分 类 号：S852.735[农业科学—基础兽医学]