日本血吸虫感染新模型小鼠IFN-γ及IL-4 mRNA的动态变化  

Dynamics changes of IFN-γ and IL-4 mRNA in the new mouse models infected with Schistosoma japonicum

作　　者：陈辉[1] 何立[1] 宗红英[1] 蒋明森[1] 杨孟祥[1] 张培喜[1] 

机构地区：[1]武汉大学基础医学院病原生物学系寄生虫学教研室,武汉430071

出　　处：《中国人兽共患病学报》2008年第4期316-318,337,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助项目(30471509);湖北省科技攻关项目(2003AA3003B04);湖北省卫生厅科研基金重点项目(JX1A11)联合资助

摘　　要：目的构建日本血吸虫感染小鼠伴随免疫新模型,通过观察比较新模型小鼠与常规感染小鼠脾细胞诱生Th1细胞因子(γ-IFN)和Th2细胞因子(IL-4)mRNA的动态变化,从分子水平探讨日本血吸虫感染小鼠伴随免疫新模型的细胞免疫机制。方法小鼠感染日本血吸虫尾蚴20d后,按300 mg/kg.d腹腔注射丙烯基硫脲,持续抑制雌虫产卵,建立日本血吸虫感染小鼠伴随免疫新模型。同时设立常规模型对照组和正常小鼠对照组。在感染后第2,3,6,9和12 w,采用RT-PCR对各组小鼠脾细胞IFN-γ和IL-4的mRNA水平进行半定量检测分析。结果新模型组与常规模型组小鼠IFN-γ和IL-4 mRNA水平变化趋势基本一致,IFN-γmRNA水平在感染后9w最强,然后逐渐下降,至第12w;IL-4 mRNA水平在感染后3w开始逐渐增强,至12w仍维持高水平。新模型组IFN-γmRNA水平在感染3w后明显高于常规模型组(P<0.05);常规模型组IL-4 mRNA水平在感染6w后明显高于新模型组(P<0.05)。正常小鼠IFN-γ和IL-4 mRNA水平较低,均无明显变化。结论日本血吸虫感染新模型小鼠与常规模型小鼠的细胞免疫功能变化趋势基本一致,表现为Th1淋巴细胞功能先升高,然后逐渐向Th2淋巴细胞功能极化,提示可溶性虫卵抗原(soluble egg antigen,SEA)不是诱导Th2功能极化的唯一因素,对细胞免疫应答由Th1向Th2功能极化有促进作用。On the base of the establishment of a new concomitant immunity mouse model fnfected with Schistosoma japonicum, the mechanism of the cell mediated immunity in this new mouse model was discussed at molecular level by observing the dynamic changes in MRNA level of Th1 cytokine （γ-IFN） and Th2 cytokine （IL-4） in spleen cells. Mice were infected with 20± 1 cercariea, and 20 days after infection, the experimental group mice were injected with allyl thiourea at the dose of 300 mg/kg every day by intraperitoneal injection. At 2, 3, 6, 9 and 12 w after infection, we practiced a semiquantitative analysis on the mRNA of IFN-7 and IL-4 in spleen cells practiced. The variance tendency of γ-IFN mRNA and IL-4 mRNA in the routine model and the new model was generally coincident. It was shown that the strap of γ-IFN mRNA reached the brightest at 9w after infection, and then decreased gradually until 12w; and the levels of IL-4 mRNA became bright from 3w after infection, increased gradually, and still maintained high at 12 w.. The strap of γ-IFN mRNA in the new model groups were obviously brighter than that in the routine model groups （P〈0.05）, while the strap of IL-4 mRNA in the routine model groups were significantly brighter than that in the new model groups 6 w after infection （P〈0. 05）. In addition, the strap of γ-IFN mRNA and IL-4 mRNA were both gloom in the normal model groups, and there were no obviously changes with it. The variance tendency of cellular immune function compared between the new concomitant immunity mouse model infected with Schistosoma japonicum and the routine infectious model was generally coincident, showing that the function of Th1 cell increases grad-ually first, and then polarize gradually to the function of Th2 cell. It suggests that even without SEA, the imago still could induce the CD4^＋ T cell polarized to Th2 cell in the new mouse model.

关 键 词：日本血吸虫 细胞因子 Γ-干扰素 白细胞介素-4 

分 类 号：R383.24[医药卫生—医学寄生虫学]