化学发光法测定氨磷汀研究  

Chemiluminescence Determination of Amifostine

作　　者：郁丰善[1] 蔡苹[2] 陈芳[1] 

机构地区：[1]华中科技大学化学与化工系,武汉430074 [2]武汉大学化学与分子科学学院,武汉430072

出　　处：《分析科学学报》2008年第2期149-152,共4页Journal of Analytical Science

基　　金：华中科技大学理科基金(No.0101013008);人才引进基金(No.0101013148)

摘　　要：实验发现，铈（Ⅳ）可以氧化氨磷汀产生弱的发光，而罗丹明6G（Rh6G）能大大增强此发光，由此建立了一种化学发光法测定氨磷汀的新方法。该方法的线性范围为1．0×10^-7～1．0×10^-5mol／L，检出限为6．4×10^-8mol／L。对1.0×10^-6mol／L的氨磷汀进行11次平行测定的相对标准偏差为2．6％。该法用于氨磷汀注射液中氨磷汀含量的测定，获得满意结果。Based on the chemiluminescence reaction of amifostine and Ce（Ⅳ ） in sulfuric acid medium significantly sensitized by rhodamine 6G, a novel chemiluminescence method for the determination of amifostine was established. The linear relationship between the intensity of chemiluminescence and the concentration of amifostine is in the range of 1.0×10^-7 ～1. 0 ×10^-5 mol/L. The detection limit is 6.4×10^-8 mol/L and the relative standard deviation is 2. 6% for 11 measurements of 1. 0 ×10^-6 mol/L standard solution. This method has been successfully applied to the determination of amifostine in amifostine for injection with a recovery range of 98.0%-104.0%. The method is simple, convenient and sensitive.

关 键 词：氨磷汀 化学发光 铈 罗丹明6G 

分 类 号：O657.39[理学—分析化学]