三黄合剂的毒理学实验研究  

Toxicity of Compound SH Chinese Medicine

作　　者：张云美[1] 潘永全[2] 韩志刚 谭祥国[3] 杨俊卿 

机构地区：[1]重庆医科大学护理学院,重庆市400016 [2]重庆医科大学实验动物中心,重庆市400016 [3]重庆工贸职业技术学院生化系,重庆市400016 [4]重庆医科大学药学院,重庆市400016

出　　处：《中国药房》2008年第12期891-893,共3页China Pharmacy

基　　金：重庆市科委自然科学基金资助项目(037998)

摘　　要：目的:观察三黄合剂对小鼠的急性毒性作用和对兔部分组织器官及肝、肾生化指标的影响。方法:按Bliss法,选择KM小鼠给予不同浓度的三黄合剂灌胃后逐日观察14d,并记录动物毒性反应、死亡数和尸检情况;给予含3%三黄合剂的饲料饲喂兔,然后在第35天取组织及血液进行观察分析。结果:发现对KM小鼠的LD50为409.88g·kg-1,LD50的95%可信限为350.80～478.91g·kg-1。经过光学显微镜观察,三黄合剂组兔的组织结构清晰,与对照组无显著变化;肝、肾生化指标也无显著差异。结论:三黄合剂的安全范围较大,LD50的剂量相当于小鼠有效剂量的40倍;对兔的心、肝、脾、肺、肾等器官形态结构和功能无显著影响,提示三黄合剂无显著的急性和亚急性毒性。OBJECTIVE： To observe the acute toxic effect of Compound SH Chinese medicine on mice and its effects on the indexes of liver and kidney in rabbits. METHODS： By Bliss method, KM mice （trial group） were selected and intragastrically administered with different concentration of compound SH Chinese medicine. Then the mice were followed for 14 days with toxic reaction, death, and autopsy conditions of the mice recorded. The rabbits （control group） were fed with 3% com- pound SH Chinese medicine-containing animal feed, from which tissue and blood samples were taken at 35 days for observation and analysis. RESULTS： Medial lethal dose （LD50） of compound SH Chinese medicine for KM mice was 409.88 g ·kg ^-l （95% confidence interval, 350.80～478.91 g·kg^-1） . The trial group showed clear microscopic structure of the tissues, showing no significant differences as compared with control group. And no significant differences were between the two groups in biochemical indicators of liver and kidney. CONCLUSION ： Compound SH Chinese medicine has a big safety margin, and its LD50is 40 times that of curative dose for mice; moreover, it has no significant influence on morphology and function of organs such as heart, liver, spleen, lung, kidney etc in animals, suggesting it has no acute and subacute toxicity.

关 键 词：三黄舍剂 小鼠 兔 毒性实验 

分 类 号：R285[医药卫生—中药学] R971[医药卫生—中医学]