HPLC法测定不同产地夏枯草中熊果酸的含量  

Determination of Ursolic Acid in Spica Prunellae from Different Habitat by HPLC

作　　者：黄丽霞[1] 贾晓斌[1] 陈彦[1] 赵呈雷[1] 封亮[1] 蔡垠[1] 

机构地区：[1]江苏省中医药研究院

出　　处：《中国药房》2008年第12期910-912,共3页China Pharmacy

基　　金：江苏省自然科学基金资助项目(BK2006155);江苏省中医药领军人才项目(2006)

摘　　要：目的:建立以高效液相色谱法测定夏枯草中熊果酸含量的方法,并检测不同产地夏枯草中熊果酸的含量。方法:色谱柱为ZorbaxSBC18(150mm×4.6mm,5μm),流动相为乙腈-磷酸盐缓冲液(pH7.6)=45∶55,检测波长为210nm,流速为1.0mL·min-1。结果:熊果酸进样量在0.1115～1.7840μg范围内与峰面积积分值呈良好的线性关系(r=0.9992);平均回收率为99.93%,RSD=0.77%(n=6)。不同产地夏枯草中熊果酸的含量在0.08%～0.18%之间。结论:不同产地夏枯草中熊果酸含量差异很大。本方法简便、准确、重现性好,可用于夏枯草的质量控制。OBJECTIVE： To establish an HPLC method for determination of the content of Ursolic acid in Spica Prunellae, and to determine the content of Ursolic acid in Spica Prunellae from different habitat. METHODS： Ursolic acid was sep- arated on Zorbax SB C18（150 mm×4.6 mm, 5 μm） with mobile phase consisted of acetonitrile- phosphate buffer （pH 7.6） = 45 ： 55 at a flow rate of 1.0 mL· min^-1. The detective wavelength was set at 210 nm. RESULTS： The linear range of Ursolic acid was 0.111 5～1.784 0μg （ r =0.999 2）. The average recovery rate of Ursolic acid was 99.93% （RSD = 0.77%, n = 6）. The content of Ursolic acid in Spica Prunellae from different habitat ranged from 0.08% to 0.18%. CONCLUSION： The content of Ursolic acid in Spica Prunellae from different habitat varied greatly. This determination method is simple, accurate and reproducible, and suitable for the quality control of Spica Prunellae.

关 键 词：高效液相色谱法 夏枯草 熊果酸 含量测定 产地 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]