HPLC法测定复方银杏叶胶囊中总黄酮苷的含量  

Determination of Total Flavonoid Glycoside in Compound GinKgo Leaf Capsule by HPLC

作　　者：李爱红[1] 胡文军[1] 

机构地区：[1]广州军区联勤部药品仪器检验所,广州市510500

出　　处：《中国药房》2008年第12期927-928,共2页China Pharmacy

摘　　要：目的:建立以高效液相色谱法测定复方银杏叶胶囊中总黄酮苷含量的方法。方法:色谱柱为Shiseido MG C18(250mm×4.6mm,5μm),流动相为甲醇-0.4%磷酸(68∶32),检测波长为378nm,以外标法定量。结果:槲皮素、山柰酚、异鼠李素的检测浓度分别在6～120μg·mL-1(r=0.99997)、3～60μg·mL-1(r=0.99998)、1～20μg·mL-1(r=0.99996)范围内与各自峰面积积分值呈良好线性关系。3批样品中的总黄酮苷含量分别为10.14、10.01、10.07mg。结论:本法快速、准确、重现性好,可用于复方银杏叶胶囊的质量控制。OBJECTIVE： To establish an HPLC method for the determination of total flavonoid glycoside in Compound ginkgo leaf capsule. METHODS： The quantitative analysis of total flavonoid glycoside was performed on Shiseido MG C18 （250 mm ×4.6 mm, 5 μm） . The mobile phase consisted of methanol - 0.4% phosphoric acid solution （68 ： 32） , The detective wavelength was 378 nm. And the quantitation was carried out using external standard method. RESULTS： The linear ranges of Quercetin, Kaempferol, and Isorhamnetin were 6～120 μg·mL^ -1（ r = 0.999 97）, 3～60 μg ·mL -1（ r = 0.999 98）, and 1～20 μg ·mL-1 （r = 0.999 96）, respectively. The contents of total flavonoid glycoside in three batches of samples were 10.14, 10.01, and 10.07 mg, respectively.CONCLUSION： The method is rapid, accurate and reproducible, and suitable fore the quality control of Compound ginkgo leaf capsule.

关 键 词：复方银杏叶胶囊 总黄酮苷 高效液相色谱法 含量测定 槲皮素 山柰酚 异鼠李素 

分 类 号：R283.65[医药卫生—中药学] R927.2[医药卫生—中医学]